摘要
目的:通过普通饲料和高脂饲料喂养大鼠,探讨建立肥胖胰岛素抵抗大鼠模型的适宜条件和时间。方法:将45只6周龄雄性SD大鼠随机分为普通饲料对照组和高脂饲料组(脂肪供能比为45%),喂养4周后剔除高脂饲料组肥胖抵抗(obesity resistance,OR)大鼠,肥胖(obese,OB)大鼠继续喂养至12周。分别于4、8、12周末进行口服葡萄糖耐量试验(oral glucose tolerance test,OGTT),12周末检测胰岛素释放、内脏脂肪、胰腺、肝脏等组织的病理变化。结果:高脂饲料喂养4周后,高脂饲料组大鼠的体质量比对照组高17.8%(P=0.001),肥胖成模率为67.6%~78.4%,OB大鼠出现糖耐量降低,OGTT 120 min血糖比对照组高27.5%(P<0.001),曲线下面积(area under the curve,AUC)比对照组高8.3%(P=0.037),79.3%的大鼠出现肥胖胰岛素抵抗;喂养8周时,OB大鼠体质量比对照组高30.4%(P<0.001),OGTT 60 min和120 min血糖分别比对照组高35.6%(P<0.001)和36.4%(P<0.001),AUC比对照组高21.7%(P<0.001),100.0%大鼠出现肥胖胰岛素抵抗;喂养12周时,OB大鼠体质量比对照组高36.9%(P<0.001),OGTT 60 min和120 min血糖分别比对照组高24.8%(P=0.001)和34.6%(P<0.001),AUC比对照组高16.1%(P=0.019),93.3%的大鼠出现肥胖胰岛素抵抗。胰岛素释放实验显示,高脂饲料组各时间点血清胰岛素均高于对照组,120 min时胰岛素浓度是对照组的6.3倍(P=0.008),胰岛和肝脏出现病理改变。结论:使用脂肪供能比为45%的高脂饲料喂养6周龄SD大鼠4周后淘汰OR大鼠,OB大鼠中出现糖耐量异常,8~12周后成模率更高。
Objective:To investigate the appropriate conditions and duration for establishing a high-fat diet-induced obesity and insulin resistance model in rats.Methods:Forty-five 6-week-old male Sprague-Dawley(SD)rats were randomly assigned into 2 groups:(1)control group(CON),(2)high-fat diet group(HFD).HFD was fed with a high-fat diet(45%kcal from fat)while CON with chow diet.After four-weeks of high-fat diet feeding,the rats of obesity resistance(OR)were eliminated according to body weight sorting,whereas obese(OB)rats were continued feeding a high-fat diet until 12 weeks.Body weight and food intake were recorded weekly.Glucose tolerance was evaluated by oral glucose tolerance test(OGTT)in 4 weeks,8 weeks and 12 weeks.At the end of 12 weeks,insulin releasing test and visceral fat mass were measured and HE staining of the liver,adipose tissue and pancreatic tissue were conducted.Results:After 4 weeks of a high-fat diet,the body weight of HFD was 17.8%higher than that of CON(P=0.001),and the rate of obesity was 67.6%-78.4%.Glucose tolerance of OB rats was impaired with a higher blood glucose concentration at 120 min(P<0.001)and a higher area under the curve(AUC,P=0.037)in OGTT compared with CON.The rate of obesity and insulin-resistance rats was 79.3%.After 8 weeks of feeding,the body weight in OB was 30.4%higher than CON(P<0.001).In OGTT,blood glucose levels at 60 min and 120 min were 35.6%and 36.4%higher than those in CON(both P<0.001),and AUC was 21.7%(P<0.001)higher than that of CON.The rate of obesity and insulin-resistance rats was 100.0%.After 12 weeks of feeding,the body weight in OB was 36.9%higher than that in CON(P<0.001).In OGTT,the blood glucose levels at 60 min and 120 min were 24.8%(P=0.001)and 34.6%(P<0.001)higher than those in CON,and AUC was 16.1%(P=0.019)higher than that of CON.The rate of obesity and insulin-resistance rats was 93.3%.The insulin releasing test showed that serum insulin concentration at each time point(0,30,60,120 min)was higher than that in CON,with a 6.3-times higher than that in CON
作者
张晓圆
郭成成
玉应香
谢岚
常翠青
ZHANG Xiao-yuan;GUO Cheng-cheng;YU Ying-xiang;XIE Lan;CHANG Cui-qing(Institute of Sports Medicine, Peking University Third Hospital, Beijing 100191, China)
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2020年第3期557-563,共7页
Journal of Peking University:Health Sciences
基金
国家重点研发计划项目(2016YFD0400603)。
关键词
胰岛素抵抗
肥胖症
膳食
高脂
模型
动物
Insulin resistance
Obesity
Diet,high-fat
Models,animal
