摘要
为明确兰属不同种兰花挥发性成分的异同,本研究以墨兰(Cymbidium sinense)、春兰(C.goeringii)、蕙兰(C.faberi)、多花兰(C.floribundum)、兔耳兰(C.lancifolium)和建兰(C.ensifolium)为实验材料,采用静态顶空气相色谱-质谱联用技术(SHS-GC-MS)和主成分分析(PCA)与聚类分析(CA)方法,对6种兰花的挥发性成分进行了分析。结果表明:6种兰花共检测出72种挥发性成分,包括醛类、萜烯、醇类、酮类、酯类、酚类、烷烃和杂环类、炔类等化合物,其中,己醛、庚醛、辛醛和壬醛等醛类物质共同存在于6种兰花;戊醛共同存在于建兰、墨兰和多花兰中,3-乙基-2-甲基-1,3-己二烯在墨兰、兔耳兰和春兰中有少量存在;苯甲醛和癸醛存在于建兰和兔耳兰,β-环柠檬醛和甜瓜醛等存在于春兰和墨兰。PCA能很好地区分6种兰花,PC1,PC2和PC3累积贡献率为95.9%,表明6种兰花挥发性成分有显著差异;CA将6种兰花分为三组,兔耳兰、春兰和建兰为一组,墨兰和多花兰为一组,蕙兰单独成组。研究结果为探究不同兰花花香的化学组成差异提供基础数据。
In order to clarify the similarities and differences of volatile components in different orchids of Cymbidium,the volatile compounds were detected by static headspace-gas chromatography-mass spectrometer(SHS-GC-MS)combined with Principal Component Analysis(PCA)and Cluster Analysis(CA)for C.sinense,C.goeringii,C.faberi,C.floribundum,C.lancifolium and C.ensifolium.The results showed that 72 volatile compounds were detected from six orchids,including aldehydes,terpenes,alcohols,ketones,esters,phenols,alkanes,heterocycles and alkynes.Hexanal,heptanal,octanal and nonanal existed in all six orchids,while pentanal existed in C.ensifolium,C.sinense and C.floribundum,and 3-ethyl-2-methyl-1,3-hexadiene was found in C.sinense,C.lancifolium and C.goeringii.Benzaldehyde and decanal existed in C.ensifolium and C.lancifolium,whileβ-Cyclocitral and melonal existed in C.goeringii and C.sinense.The six orchids could be distinguished well by PCA,with accumulative contribution rate 95.9%of PC1,PC2 and PC3,indicating that volatile compounds of six orchids were significantly different.Six orchids were clustered into three groups by cluster analysis.C.lancifolium,C.goeringii and C.ensifolium formed the first group,and C.sinense and C.floribundum were clustered in the second group,while C.faberi was clustered in the third group.The results provided basic datas for exploring the chemical composition difference of different orchid fragrance.
作者
周雅莲
王文静
吴栋
薛阿辉
崔萌
黄学勇
罗丽萍
ZHOU Yalian;WANG Wenjing;WU Dong;XUE Ahui;CUI Meng;HUANG Xueyong;LUO Liping(School of Life Sciences,Nanchang University,Nanchang 330031,China;State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)
出处
《南昌大学学报（理科版）》
CAS
北大核心
2020年第1期49-55,62,共8页
Journal of Nanchang University(Natural Science)
基金
国家自然科学基金资助项目(31772067)
江西省优势科技创新团队建设专项计划(20171BCB24002)
南昌大学研究生创新专项资金项目(CX2018108)。
