摘要
[目的]为某规模化猪场猪伪狂犬病病毒野毒株感染防控工作提供科学的免疫防控方案。[方法]对某发病猪群(采用Bartha-K61经典毒株疫苗进行了猪伪狂犬病免疫)进行流行病学调查,并采用ELISA方法检测其猪伪狂犬病gB抗体和gE抗体水平。[结果]根据调查、解剖和血清抗体检测结果,初步确诊该发病猪群为猪伪狂犬病野毒感染。通过采取紧急免疫伪狂犬病HB2000毒株疫苗、调整免疫程序和配合药物治疗等措施,育肥猪死亡率从2.56%降低至0.60%;除了4周龄及12周龄猪群外,其余猪群伪狂犬病野毒抗体水平全部下降;种公猪、8周龄、10周龄、14周龄猪群伪狂犬病野毒抗体阳性率均为0。[结论]Bartha-K61经典毒株疫苗并不能提供完全的保护力,通过紧急免疫与流行毒株同源性较高的HB2000毒株疫苗,加强生物安全管理工作,能够有效控制猪伪狂犬病野毒感染。
[Objective]To provide a scientific immunization program for the prevention and control of wild-type pseudorabies virus infection in a large-scale pig farm.[Method]Epidemiological investigation was carried out in the infected pigs that immunized with the classical strain of Bartha-K61,and the serum levels of gB and gE antibodies were detected by ELISA.[Result]The epidemiological investigation,pathological autopsy and serum antibody detection preliminarily confirmed that the affected pigs were infected by wild-type pseudorabies virus.Through the emergency immunization of vaccine prepared with pseudorabies virus HB2000 strain,adjustment of immunization procedures and implementation of drug health care measures,the mortality rate of finishing pigs was reduced from 2.56%to 0.60%;except for 4-week-old and 12-week-old pigs,the antibody levels against the wild-type pseudorabies virus of the other pigs were all decreased;the positive rate against the wild-type pseudorabies virus of the stud boars and 8-week-old,10-week-old and 14-week-old pigs was 0.[Conclusion]The Bartha-K61 vaccine cannot provide the complete protection.It can effectively control the infection of wild-type pseudorabies virus by emergency immunization of vaccine prepared with HB2000 strain that sharing high homology with the prevalent viral strain.Furthermore,strengthening of the biological safety measures is also helpful and urgent.
作者
王礼伟
周刚
王萍
李鹏
WANG Li-wei;ZHOU Gang;WANG Ping;LI Peng(Huaiyin Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province,Huaian 223001,China;Animal Husbandry and Veterinary Medicine Service Station of Matou Town of Huaiyin District of Huaian City,Huaian 223345,China;Animal Husbandry and Veterinary Medicine Service Station of Huaigao Town of Huaiyin District of Huaian City,Huaian 223341,China)
出处
《畜牧与饲料科学》
2020年第3期96-101,共6页
Animal Husbandry and Feed Science
基金
江苏省政策引导类计划(苏北科技专项)科技帮扶项目(SZ-HAZ2019004)
江苏省政策引导类计划(苏北科技专项)科技帮扶项目(SZ-HAZ201800)
淮安市农业科学研究院科研发展基金项目(HYN201920)。
关键词
伪狂犬病毒野毒
流行病学调查
酶联免疫吸附试验
野毒感染
猪
wild-type pseudorabies virus
epidemiological investigation
ELISA
infection of wild-type viral strain
pigs
