摘要
目的探讨麝香通心滴丸对自发性高血压大鼠血管内皮的作用及其对Rho/ROCK信号通路的影响。方法SPF级自发性高血压大鼠20只及同类正常血压大鼠10只,随机分为正常血压组(对照组)、自发性高血压模型组(SHR组),麝香通心滴丸组(STX组),每组10只。观测各组大鼠血压变化;检测大鼠血清中一氧化氮(NO)、超氧化物歧化酶(SOD)及丙二醛(MDA)水平;酶联免疫吸附实验(ELISA)检测血管中内皮素-1(ET-1)、血管内皮生长因子(VEGF)含量;实时PCR检测血管组织中ET-1及VEGF mRNA的表达;利用Western blotting检测血管组织中Rho/ROCK信号通路相关蛋白RhoA、ROCK及MLC蛋白的表达。结果与SHR组比较,STX组大鼠血压明显降低;SOD活性及NO含量显著增加,而MDA水平却明显降低;ET-1及VEGF蛋白及mRNA水平明显降低,差异均有统计学意义(均P<0.05);同时,与SHR组比较,STX组大鼠血管组织RhoA、ROCK及MLC蛋白表达明显降低,差异均具有统计学意义(P<0.05)。结论麝香通心滴丸对自发性高血压大鼠模型的血管内皮有保护作用,可能与抑制Rho/ROCK信号通路相关蛋白表达有关。
Objective To investigate the protective effect of musk tongxin dropping pills on the vascular endothelium in spontaneously hypertensive rats(SHR)and examine the effect on the Rho/ROCK signaling pathway.Methods SHR and normal blood pressure rats were randomly divided into normal blood pressure(control group,n=10),spontaneous hypertension model group(SHR group,n=10),and musk tongxin dropping pill groups(STX,n=10).For each animal group,the blood pressure changes were recorded.The rat serum levels of nitric oxide(NO),superoxide dismutase(SOD),and malondialdehyde(MDA)were detected using a colorimetric kit.In the blood vessels,endothelin-1(ET-1)and vascular endothelial growth factor(VEGF)levels were detected using the enzyme-linked immunosorbent assay(ELISA);real-time PCR was used to detect the expression of ET-1 and VEGF mRNA in vascular tissues.Additionally,in the vascular tissues,the expression of RhoA,ROCK,and MLC proteins in the Rho/ROCK signaling pathway was detected using Western blotting.Results Compared with the SHR group,the blood pressure of the STX group was significantly decreased.The SOD activity and NO content were significantly increased,while the MDA level was significantly decreased.The ET-1 and VEGF protein expression and mRNA levels were significantly decreased;all differences were statistically significant(P<0.05).Simultaneously,compared with the SHR group,RhoA,ROCK,and MLC protein expressions were significantly decreased in the vascular tissue of the STX group,and the difference was statistically significant(all P<0.05).Conclusion Musk tongxin dropping pills demonstrated a protective effect on the vascular endothelium of the SHR model,which could be related to the inhibition of the Rho/ROCK signaling pathway-related protein expression.
作者
李立杰
葛华
陈曦
鲁伟
王月
陈克研
LI Lijie;GE Hua;CHEN Xi;LU Wei;WANG Yue;CHEN Keyan(Department of Cardiology,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China;Department of Laboratory Animal Science,China Medical University,Shenyang 110122,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2020年第6期485-489,504,共6页
Journal of China Medical University
基金
国家自然科学基金(81471121)。
