摘要
为准确揭示节节麦醇溶蛋白的组成情况,对不同地理分布的212份节节麦的种子蛋白进行酸性聚丙烯酰胺凝胶电泳(A-PAGE)分析。由于麦醇溶蛋白现有的"数字字母命名"系统并不具备广泛的普适性,将普通小麦--‘中国春’(对照)第1条谱带的迁移率作为标准,其他供试材料的谱带与它的比值作为相对迁移率来确定每条醇溶蛋白条带的位置。结果显示,212份节节麦材料中共存在422种不同迁移率的谱带。所有条带可分为ω、γ、β和α4个区,α区的多态性条带最多,达34.70%。多态性条带品种间遗传相似系数(GS)的变幅为0~0.58,遗传变异较大。聚类分析结果表明,节节麦可能是以伊朗为中心向四周各地扩散传播。中国境内的节节麦至少存在3种类型。基于相对迁移率的表示方法,无需除‘中国春’以外的其他对照材料,即可有效区分A-PAGE胶上距离较近的蛋白条带,为不同麦类作物醇溶蛋白的研究提供统一标准。
To reveal the composition of gliadinin Aegilops tauschii cosson,acid-polyacrylamide gel electrophoresis(A-PAGE)analysis was performed in 212 accessions of Ae.tauschii from different geographical distributions.Since the present number-letter naming system for gliadin is not widely universal,the mobility ratio of the first band of common wheat-‘Chinese Spring’(control)was used as the standard,and the mobility ratio of gliadin bands of other materials was taken as the relative mobility ratio to determine the position of each gliadin band.The results showed that a total of 422 bands with different mobilities were isolated from 212 materials.All bands were divided into four regions ofω,γ,βandα.There were the most polymorphism inαregion,which accounted for 34.70%.The genetic similarity coefficient(GS)of polymorphic bands varied from 0 to 0.58,which its significant genetic variation resided within the materials.Cluster analysis indicated that the Ae.tauschii may be origined from Iran.There were at least three types of Ae.tauschii in China.The relative mobility-based representation method used in this study just required‘Chinese Spring’as reference and could distinguish protein bands effectively and easily on A-PAGE gels.Also this method couldprovide a uniform standard for the gliadin of different Triticeae crops.
作者
叶发慧
察艳艳
冯美玲
李响
刘瑞娟
曹东
张波
刘宝龙
陈文杰
张怀刚
YE Fahui;CHA Yanyan;FENG Meiling;LI Xiang;LIU Ruijuan;CAO Dong;ZHANG Bo;LIU Baolong;CHEN Wenjie;ZHANG Huaigang(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Xining 810008,China;University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Crop Molecular Breeding,Qinghai,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Qinghai University,Xining 810016,China;Qinghai Normal University,Xining 810008,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2020年第6期829-841,共13页
Acta Agriculturae Boreali-occidentalis Sinica
基金
青海省应用基础研究(2018-ZJ-701)
国家重点研发计划(2016YFD0100500)
中国科学院战略性先导科技专项(XDA24030102)。
