摘要
【目的】探讨在低温条件下公主岭霉素对水稻幼苗生长及耐冷相关基因表达、抗逆防御酶活性的影响,明确施用公主岭霉素后育苗期水稻耐冷性的变化。【方法】以‘吉粳88’为试验材料,经公主岭霉素水浸提液浸种引发后播种,调查不同温度下稻种萌发和幼苗生长状况并计算稻种发芽临界温度。将农抗“769”固体发酵物干燥粉碎后添加到水稻育苗基质中,以‘吉粳88’为试验材料,在立针期模拟倒春寒生境对水稻幼苗施加冷胁迫处理,在处理后逐渐缓慢升温并于1—8 d内连续采样,升温至28℃后每隔7 d采样一次;以‘吉宏6号’为试验材料,大棚育苗待水稻幼苗长至一叶一心期采样;采用实时荧光定量PCR(RT-qPCR)技术,分析模拟生境及大棚育苗环境中幼苗叶片耐冷相关基因OsNAC6、OsSADMC、OsETR4、OsZFP151的表达状况,并检测移栽前大棚秧苗叶片中防御酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)活性的变化。【结果】公主岭霉素能够提高稻种发芽率和发芽指数,缩短发芽时间,其对幼苗生长的促进作用随温度的降低而逐渐明显,G-500X表现最好,使稻种萌发的临界温度降低了4.09%,并以该浓度为基础进行后续的试验研究。光照培养箱低温模拟倒春寒气候条件,低温胁迫下公主岭霉素可调控OsNAC6、OsSADMC、OsETR4表达量的提升,并通过加快响应速度,实现快速反应以应对低温胁迫,对照处理主要通过调控OsZFP151的响应速度和表达量提升应对低温胁迫,但公主岭霉素处理下的水稻幼苗该基因表达量亦得到提升。水稻幼苗在立针期受到低温胁迫后,公主岭霉素处理的幼苗在低温胁迫后OsNAC6、OsSADMC、OsETR4表达量峰值出现时间较对照早1—2 d,且分别比对照提高38.57%、74.66%、130.61%;OsZFP151最高表达量的时间较对照迟2 d,但最高表达量比对照提高了34.91%。大棚�
【Objective】The objective of this study is to investigate the effects of gongzhulingmycin on rice seedling growth,cold tolerance genes expression and defense enzyme activities based on chilling stress exposure,and to elucidate the change in cold tolerance induced by gongzhulingmycin at the breeding stage of rice.【Method】Rice variety‘Jijing88’was used as the experimental material,the seed germination and seedling growth were investigated under different temperatures and the critical temperature of seed germination was calculated after seed priming by gongzhulingmycin.The solid fermentation product of gongzhulingmycin was powdered and added into the rice seedling substrate before sowing.Taking‘Jijing88’as the experimental material,the rice seedlings were subjected to cold stress treatment in simulated cold environment in late spring at the needle appearance stage of rice.Gradually warmed up and sampled continuously within 1-8 d after treatment and sampled every 7 days when plant temperature raised to 28℃.Taking‘Jihong 6’as the experimental material,the rice seedlings were raised in the greenhouse and sampled when the seedlings grew to one leaf at a time.The expression of 4 cold tolerance genes(OsNAC6,OsSADMC,OsETR4 and OsZFP151)under the simulated cold environment in late spring and greenhouse seedling environment was analyzed by real-time quantitative PCR(RT-qPCR).The changes of defense enzyme activities such as superoxide dismutase(SOD),catalase(CAT),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO)in greenhouse seedlings before transplanting were detected.【Result】The gongzhulingmycin could improve seed germination rate and germination index,shorten mean germination time,and its promoting effect on seedling growth was gradually obvious with the decrease of temperature.G-500x showed the best performance,which reduced the critical temperature of rice seed germination by 4.09%,and the subsequent experimental study was carried out based on this concentration.At the condition of low tem
作者
安俊霞
赵宇
张正坤
史海鹏
纪东铭
曹洪翼
杜茜
李启云
AN JunXia;ZHAO Yu;ZHANG ZhengKun;SHI HaiPeng;JI DongMing;CAO HongYi;DU Qian;LI QiYun(Institute of Plant Protection,Jilin Academy of Agricultural Sciences/Jilin Key laboratory of Agricultural Microbiology/Key Laboratory of Integrated Pest Management on Crops in Northeast,Ministry of Agriculture and Rural Affairs,Changchun 130033;College of Plant Protection,Jilin Agricultural University,Changchun 130118;Agricultural Technology Extension Station of Yitong Manchu Autonomous County,Yitong 30700,Jilin;Plant Protection Station of Siping City,Siping 136000,Jilin;Life Science and Technology Academy,Harbin Normal University,Harbin 150080)
出处
《中国农业科学》
CAS
CSCD
北大核心
2020年第11期2195-2206,共12页
Scientia Agricultura Sinica
基金
国家重点研发计划(2017YFD0201104)
吉林省农业科技创新工程自由创新项目(CXGC2018ZY022)
吉林省农业科技创新工程研究生基金(安俊霞2018)。
关键词
公主岭霉素
水稻
耐冷性
基因表达
防御酶活性
gongzhulingmycin
rice
cold tolerance
gene expression
defense enzyme activity
