摘要
目的探讨结肠相关背根神经节(dorsal root ganglia, DRG)中P2X3受体参与艾灸足三里穴缓解肠易激综合征(irritable bowel syndrome, IBS)大鼠内脏痛的效应与作用机制。方法清洁级雄性SD新生大鼠随机分为正常组和造模组,采用直结肠球囊扩张(colorectal dilatation, CRD)的方法诱导IBS慢性内脏痛模型。模型鉴定成功后再将造模组大鼠随机分为模型组、艾灸组、P2X3受体拮抗剂组、生理盐水组,艾灸组取双侧足三里穴进行艾灸干预,P2X3受体拮抗剂组进行鞘内注射P2X3受体拮抗剂干预,生理盐水组进行鞘内注射生理盐水干预。运用行为学、免疫组织化学、Western blot、RT-qPCR等方法观察结肠相关背根神经节中P2X3受体在艾灸缓解IBS大鼠内脏痛中的作用。结果与正常组比较,模型组大鼠在20、40、60、80 mmHg不同压力强度CRD刺激下内脏痛行为学评分明显升高,结肠相关DRG中P2X3受体蛋白及其mRNA表达明显增加(均P<0.05);与模型组比较,艾灸组大鼠在20、40、60、80 mmHg不同压力强度CRD刺激下内脏痛行为学评分明显降低,结肠相关DRG中P2X3受体蛋白及其mRNA表达明显减少(均P<0.05)。结论艾灸能够调节IBS大鼠结肠相关DRG中异常增高的P2X3受体蛋白及mRNA表达,可能是艾灸缓解IBS大鼠内脏痛外周敏化的作用机制。
Objective To discuss the effect of P2X3 from colon-associated dorsal root ganglia(DRG)participating in moxibustion at Zusanli(ST36)to relieve visceral pain in irritable bowel syndrome(IBS)rats.Method Clean-grade neonatal male Sprague-Dawley(SD)rats were randomized into a normal group and a model group.IBS chronic visceral pain model was established using neonatal rat colorectal distending(CRD)method.When the model was confirmed a success,the model group rats were randomized into a model group,a moxibustion group,a P2X3 receptor antagonist group and a normal saline group.Bilateral Zusanli points were selected to receive moxibustion intervention in the moxibustion group;the P2X3 receptor antagonist group received intrathecal injection of P2X3 receptor antagonist;the normal saline group received intrathecal injection of normal saline.Behavioral study,and immunohistochemistry,Western Blot(WB),and real-time quantitative polymerase chain reaction(RT-qPCR)methods were adopted to observe the effect of P2X3 receptor from colon-associated DRG in moxibustion treatment to relieve visceral pain in IBS rats.Result Moxibustion at Zusanli significantly reduced the visceral pain-related behavioral score under CRD pressure of 20,40,60 and 80 mmHg in IBS rats.Moxibustion at Zusanli down-regulated the protein and mRNA expressions of P2X3 from colon-associated DRG in IBS rats.Conclusion Moxibustion can down-regulate the abnormally increased P2X3 receptor protein and mRNA expressions from colon-associated DRG in IBS rats,which may be the mechanism of moxibustion in alleviating the peripheral sensitization of visceral pain in IBS rats.
作者
张方
周云
吴焕淦
翁志军
张智英
赵敏
许琼
刘慧荣
周次利
ZHANG Fang;ZHOU Yun;WU Huan-gan;WENG Zhi-jun;ZHANG Zhi-ying;ZHAO Min;XU Qiong;LIU Hui-rong;ZHOU Ci-li(Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《上海针灸杂志》
2020年第6期775-782,共8页
Shanghai Journal of Acupuncture and Moxibustion
基金
上海市卫生和计划生育委员会科研课题(20174Y0015)
上海市青年科技英才扬帆计划项目(18YF1421600)
国家自然科学基金面上项目(81873367,81873377)
上海市卫生健康委员会项目(201940064)。
关键词
灸法
艾条灸
肠易激综合征
内脏痛
P2X3受体
穴
足三里
大鼠
Moxibustion
Moxa-stick moxibustion
Irritable bowel syndrome
Visceral pain
P2X3 receptor
Point
Zusanli(ST36)
Rats
