摘要
目的分析住院患者生物标本中检出金黄色葡萄球菌肠毒素分型特点及其耐药性。方法使用酶联免疫吸附法对我院2018年1月-2019年6月住院患者送检痰液、血液、尿液、分泌物、胸腹水、引流液、肺泡灌洗液等标本中分离出的226株金黄色葡萄球菌进行肠毒素SEA^SEE分型;仪器法进行药敏试验,纸片扩散法(K-B法)验证。结果226株金黄色葡萄球菌中,其中耐甲氧西林金黄色葡萄球菌(MRSA)92株(40.71%),甲氧西林敏感金黄色葡萄球菌(MSSA)134株(59.29%);其中184株细菌检出肠毒素,阳性率为81.42%,其中MRSA85株(92.39%)检出肠毒素,MSSA99株(73.88%)检出肠毒素。金黄色葡萄球菌对青霉素、四环素、环丙沙星、左氧氟沙星、克林霉素、红霉素等抗菌药物整体耐药率超过了50%;对万古霉素、利奈唑胺、替加环素的敏感率为100%。结论金黄色葡萄球菌特别是MRSA肠毒素检出率高,对多种抗菌药物呈现高耐药性,临床重点科室应加强措施,防止院内感染。
Objective To analyze the enterotoxin typing characteristics and drug resistance of Staphylococcus aureus which is detected from biological specimen of hospitalized patients.Methods Enzyme-linked immunosorbent assay(ELISA)was used to detect enterotoxin SEA-SEE typing of Staphylococcus aureus from 226 cases of sputum,blood,urine,secretions,pleural and ascites,drainage fluid,alveolar lavage fluid,etc.in hospitalized patients from January 2018 to June 2019 in our hospital;Instrumental method for drug susceptibility test,paper diffusion method(KB method)verification.Results Among 226 strains of Staphylococcus aureus,92 strains of methicillin-resistant Staphylococcus aureus(MRSA)(40.71%)and 134 strains of methicillin-sensitive Staphylococcus aureus(MSSA)(59.29%);184 strains of bacteria were detected.The positive rate of enterotoxin was 81.42%.Among them,85 strain(92.39%)of MRSA detected enterotoxin,99 strain(73.88%)of MSSA detected enterotoxin.The overall resistance rate of Staphylococcus aureus to Penicillin,Tetracycline,Ciprofloxacin,Levofloxacin,Clindamycin,Erythromycin and other antibacterial drugs exceeded 50%;for Vancomycin,Linezolid,Tigecycline The sensitivity rate is 100%.Conclusion Staphylococcus aureus especially MRSA has a high detection rate of enterotoxin and is highly resistant to various antibacterial drugs.The key clinical departments should strengthen measures to prevent nosocomial infections.
作者
伊怀文
Yi Huaiwen(Department of Medical Laboratory,The First People’s Hospital of Jining,Jining,Shandong,272100)
出处
《口岸卫生控制》
2020年第1期16-19,共4页
Port Health Control
关键词
金黄色葡萄球菌
肠毒素
耐药性
院内感染
Staphylococcus aureus
Enterotoxin
Drug resistance
Nosocomial infection
