摘要
目的:利用近红外光谱法,从整体上对不同产地的覆盆子药材进行了快速无损鉴别研究。方法:采用近红外光谱技术不经任何化学处理,对原始样品直接检测,获取样品的全部完整的光谱信息,通过结合聚类分析、相似度计算和主成分分析多种模式识别技术对样品进行定性分析,实现了不同产地覆盆子药材的快速无损鉴别。结果:虽然不同产地覆盆子所含化学成分不尽相同,但是相似的组成在近红外光谱图上显示出极其的相似性,在1401.4~2252.5 nm的波长区间,借助于聚类分析的模式识别方法能够用于覆盆子产地的快速鉴别,这与相似度计算以及主成分分析的结果一致。结论:该方法准确可信、快速无损、采样少,它为中药材产地的分析鉴别提供了新的方法参考,应用前景良好。
Objective:To quickly identify Rubus Chingii Hu,the medicinal material from different regions without damage using near-infrared diffuse reflectance spectrometry.Methods:The near-infrared diffuse reflectance spectrometry was used to detect the original samples directly without any chemical treatment to obtain the complete spectral information of the samples.Through the combination of cluster analysis,similarity calculation,and principal component analysis,multiple pattern recognition technologies,the samples were qualitatively analyzed to achieve the rapid and non-destructive identification of Rubus Chingii Hu from different regions.Results:Although the chemical components of Rubus Chingii Hu from different regions were different,the similar components showed extremely similar on the near-infrared spectrum.In the wavelength region of 1401.4~2252.5 nm,the pattern recognition method based on the cluster analysis could be used for rapid identification of Rubus Chingii Hu origin,which was consistent with the results of similarity calculation and principal component analysis.Conclusion:The method is accurate,reliable,fast and nondestructive,and with few samples.It provides a new method reference for the analysis and identification of the origin of traditional Chinese medicine,and has a good application prospect.
作者
高鸿彬
瞿敏
刘浩
相秉仁
GAO Hongbin;QU Min;LIU Hao;XIANG Bingren(Department of Pharmacy,Baoshan Branch of Ren Ji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200444,China;Guangdong Food and Drug Vocational College,Guangzhou 510520,China;China Pharmaceutical University,Nanjing 210009,China)
出处
《世界中医药》
CAS
2020年第10期1386-1390,共5页
World Chinese Medicine
基金
军队医疗机构制剂标准提高计划科研专项课题(14ZJZ02-3)
广东省自然科学基金项目(10151052005000003)。
关键词
近红外漫反射光谱法
覆盆子
快速无损鉴别
主成分分析
相似度计算
聚类分析
Near-infrared diffuse reflectance spectrometry
Rubus Chingii Hu
Rapid and nondestructive identification
Principal component analysis
Similarity calculation
Cluster analysis
