摘要
目的:运用网络药理学的研究方法探究牛大力治疗类风湿性关节炎(RA)的作用机制。方法:从中药系统药理学数据库与分析平台(TCMSP)及文献资料中检索和筛选牛大力活性成分,并通过TCMSP和Swiss Target Prediction数据库预测其潜在作用靶点;同时,从OMIM、Mala Cards、Therapeutic Target Database数据库中搜索RA的相关靶点,采用Cytoscape 3.6.0软件构建活性成分-靶点-RA网络。此外,利用String数据库和DAVID数据库进行蛋白相互作用分析、基因本体(GO)富集分析和KEGG通路富集分析。结果:本研究筛选出43个牛大力活性成分包括补骨脂黄酮(17)、甘草素(18)、槲皮素(15)、高丽槐素(1)等关键成分,26个关键靶点包括肿瘤坏死因子(TNF)、血管内皮生长因子A(VEGFA)、信号转导和转录激活因子3(STAT3)、白细胞介素-2(IL2)、前列腺素G/H合成酶2(PTGS2)等,其中涉及到以肿瘤坏死因子信号通路为主的炎症相关通路。结论:本研究通过网络药理学研究方法预测了牛大力治疗RA的相关活性成分及潜在作用机制,为深入研究牛大力治疗RA作用机制提供一个新方向。
Objective:To explore the mechanism of Millettia speciosa Champ.in the treatment of rheumatoid arthritis(RA)based on network pharmacology.Methods:The active components of Millettia speciosa Champ.were searched and screened from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and literature data,and the potential action targets of the active components were predicted by TCMSP and SwissTargetPrediction databases.At the same time,the related targets of RA were searched from OMIM,MalaCards,and Therapeutic Target Database.The active component-target-RA network was constructed by Cytoscape 3.6.0 software.In addition,String database and DAVID database were used for protein interaction analysis,gene ontology(GO)enrichment analysis,and KEGG pathway enrichment analysis.Results:In this study,43 active components of Millettia speciosa Champ.were screened,such as bavachin(17),glycyrrhizin(18),quercetin(15),Maackiain(1);26 key targets of RA for instance tumor necrosis factor(TNF),vascular endothelial growth factor A(VEGFA),signal transduction and transcriptional activator 3(STAT3),interleukin-2(IL2),prostaglandin G/H synthetase 2(PTGS2)etc,which involved in inflammation-related pathways that mainly based on tumor necrosis factor signaling pathways.Conclusion:In this study,the related active components of Millettia speciosa Champ.and its potential mechanism in the treatment of RA were predicted by network pharmacology,which provides a new sight for the further study of the mechanism of Millettia speciosa Champ.in the treatment of RA.
作者
莫祎祎
苏志恒
梁永红
Mo Yiyi;Su Zhiheng;Liang Yonghong(Pharmaceutical College,Guangxi Medical University,Nanning 530021,China)
出处
《广西医科大学学报》
CAS
2020年第5期843-849,共7页
Journal of Guangxi Medical University
基金
广西自然科学基金面上项目资助(No.2018GXNSFAA138096)
国家自然科学基金资助项目(No.81560626)。
关键词
牛大力
类风湿性关节炎
网络药理学
Millettia speciosa Champ.
rheumatoid arthritis
network pharmacology
