摘要
Leptospirillum ferriphilum YSK was added to a native consortium of bioleaching bacteria including Acidithiobacillus caldus,A.thiooxidans,A.ferrooxidans,Sulfobacillus thermosulfidooxidans,Acidiphilium spp.,and Ferroplasma thermophilum cultured in modified 9K medium containing 0.5%(W/V)pyrite.The bioleaching efficiency markedly increased.Changes in community structure and gene expression were monitored with real-time PCR and functional gene arrays.Dynamic changes that varied in different populations in the consortium occurred after the addition of L.ferriphilum YSK,with growth of A.caldus S1,A.thiooxidans A01,Acidiphillum spp.DX1-1 promoted the growth of Ferroplasma L1,inhibited that of S.thermosulfidooxidans ST,and exerted little effect on that of A.ferrooxidans CMS.Genes encoding ADP heptose,phosphoheptose isomerase,glycosyltransferase,biotin carboxylase,and protoheme ferrolyase from L.ferriphilum,acetyl-CoA carboxylase from Acidiphillum spp.,and doxD from A.caldus were up-regulated in 0-20 h.Genes encoding lipid A disaccharide synthase LpxB,glycosyl transferase,and ADP heptose synthase from A.ferrooxidans were up-regulated in 0-8 h and then down-regulated in 8-20 h.Genes encoding ferredoxin oxidoreductase from Ferroplasma sp.were up-regulated in 0-4 h,down-regulated in 4-16 h,and again up-regulated in 16-20 h.CbbS from A.ferrooxidans was down-regulated in 0-20 h.
在含有0.5%(W/V)黄铁矿的改良9K培养基中,添加Leptospirillum ferriophilum YSK到天然生物浸出菌群中,包括Acidithiobacillus caldus,A.thiooxans,Sulfobacillus thermosfidooxans,Acidiphilium spp.和Ferrodium thermophilum,浸出率明显提高,并用实时荧光定量PCR和功能基因芯片监测群落结构和基因表达的变化。加入L.ferriophilum YSK后群落内不同种群间的动态变化不同,促进A.caldus S1,A.thiooxans A0,Acidiphillum spp.DX1-1的生长,抑制了Ferroplasma L1和S.Thermosulfidooxidans ST的生长,但对A.ferrooxidans CMS的影响不大。功能基因芯片分析发现:来自L.ferriphilum的编码ADP heptose,phosphoheptose isomerase,glycosyltransferase,biotin carboxylase和protoheme ferrolyase的基因,以及来自Acidiphillum spp编码的acetyl-Co A carboxylase和来自A.caldus的dox D基因在引种后0~20 h内表达水平上调;来自A.ferrooxidans编码的lipid A disaccharide synthase Lpx B,glycosyl transferase和ADP heptose synthase的基因在引种后0~8h表达水平上调,在8~20h下调;来自Ferroplasma sp.编码的ferredoxin oxidoreductase的基因在引种后0~4 h表达水平上调,而在4~16 h表达水平下调,在16~20 h表达上调;来自A.ferrooxidans的Cbb S基因在引种0~20 h表达水平下调。
作者
SHEN Li
WANG Jun-jun
LIU Hong-wei
YIN Hua-qun
LIU Xue-duan
QIU Guan-zhou
LIU Yi
申丽;王俊俊;刘宏伟;尹华群;刘学端;邱冠周;刘毅(School of Minerals Processing and Bioengineering,Central South University,Changsha 410083,China;Key Laboratory of Biohydrometallurgy of Ministry of Education,Central South University,Changsha 410083,China;Key Laboratory of Agro-ecological Processes in Subtropical Regions and Taoyuan Station of Agro-ecology Research,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China)
基金
Projects(51604308,41771300,41301274)supported by the National Natural Science Foundation of China
Project(2017QNCXTD_GTD)supported by the Youth Innovation Team Project of Institute of Subtropical Agriculture,Chinese Academy of Sciences
Project(2017YFD0202000)supported by the National Key Research and Development Program of China
Project(2020GDASYL-20200402001)supported by the special Project of Science and Technology Development,China。
