摘要
为探究某动物园西部白眉长臂猿死亡的可能原因,无菌采集病死白眉长臂猿肺脏组织进行细菌分离鉴定。通过分离纯化、培养特性观察、革兰染色镜检、生化试验以及16S rDNA克隆测定对分离菌株进行鉴定;进一步研究分离株的致病性、耐药性,并进行超广谱β-内酰胺酶耐药基因检测及测序分析。结果表明,分离得到1株革兰阴性小杆菌,命名为J181121,该分离菌的培养及生化特性符合支气管败血波氏杆菌,其16S rDNA基因序列与Genbank中支气管败血波氏杆菌(登录号:CP018761.1)的同源性为99%,对小鼠致病力强。药敏试验结果显示,分离株对β-内酰胺类抗生素耐药,PCR检测到超广谱β-内酰胺酶耐药基因bla-SHV。
In order to explore the possible death cause of the Hylobates hoolock in a zoo,the bacteria was isolated and identified from the lungs.Isolation and purification,culture characteristics observation,gram staining microscopy,biochemical test and 16S rDNA cloning were conducted to identify the isolated strains.The pathogenicity and drug resistance of the isolates were further studied,and the ultrabroad spectrum beta-lactamase resistance genes were tested and sequenced.The result showed that a strain of gram-negative bacillus was successfully isolated,which was named J181121.The isolate was tested for pathogenicity and drug resistance,and further extended-spectrumβ-lactamase resistance genes were detected and sequenced.The culture and biochemical characteristics of J181121 were consistent with Bordetella bronchiseptica,and the 16S rDNA gene sequence of this isolate showed 99%homology with B.bronchiseptica(accession number:CP018761.1)in genbank.And it was highly pathogenic to mice.The drug sensitivity test showed that the isolate was resistant toβ-lactam antibiotics,and the extended-spectrumβ-lactamase gene bla-SHV could be amplified by PCR.
作者
姜睿姣
张鹏飞
王印
姚学萍
杨泽晓
罗燕
JIANG Ruijiao;ZHANG Pengfei;WANG Yin;YAO Xueping;YANG Zexiao;LUO Yan(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Key Laboratory of Animal Disease and Human Health of Sichuan Province,Chengdu 611130,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2020年第5期668-674,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家农业产业技术体系四川兽药创新团队专项(CARS-SVDIP)。
关键词
白眉长臂猿
支气管败血波氏杆菌
分离鉴定
超广谱Β-内酰胺酶
Hylobates hoolock
Bordetella bronchiseptica
Isolation and identification
Extended-spectrumβ-lactamases
