摘要
目的探讨脑源性神经营养因子(BDNF)对人冠状动脉内皮细胞(human coronary artery ebditgekuak cells,HCAEC)功能的影响及其作用机制。方法将HCAEC分为2组,其中一组添加BDNF(50 ng/μL)作为实验组,另一组不做其他处理作为对照组。采用CCK8法和平板克隆形成实验分别检测2组细胞的增殖能力和克隆能力,采用Western blot检测2组细胞中PI3K/Akt/mTOR信号通路、ERK信号通路及Wnt/β-catenin信号通路相关蛋白水平。结果与对照组相比,实验组细胞的增殖能力和克隆能力均显著提高(P <0. 05)。Western blot结果表明,实验组细胞中的磷酸化Akt(p-Akt)和磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)水平显著升高(P <0. 05),并且Wnt蛋白家族1(Wnt1)、跨膜受体卷曲蛋白1(Frizzled1)和胞浆调节散乱蛋白1(Dsh)的表达水平也明显增加(P <0. 05),磷酸化细胞外调节蛋白激酶(p-ERK1/2)水平升高(P <0. 05)。结论BDNF促进了HCAEC的细胞增殖和克隆形成能力,PI3K/Akt/mTOR信号通路、ERK信号通路和Wnt/β-catenin信号通路可能参与其中。
Objective To investigate the effect of brain-derived neurotrophic factor(BDNF)on the cell function of human coronary artery endothelial cells(HCAEC).Methods HCAECs were divided into two groups,one group was treated with BDNF(50 ng/μL) as experimental group,the other group without any treatment was the control group.CCK8 assay and plate clone formation assay were adopted to detect cell proliferation and cell clone formation,respectively.The protein levels of PI3 K/Akt/mTOR signaling pathway,ERK signaling pathway and Wnt/β-catenin signaling pathway were determined by Western blot.Results Compared with the control group,the proliferation and clone formation of experimental group were significantly increased(P <0.05).The Western blot test showed that the expression of p-Akt and p-mTOR was induced by BDNF(P <0.05).And the expression of Wnt/β-catenin signaling pathway related-proteins was also induced by BDNF(P <0.05).The protein of p-ERK1/2 was increased(P <0.05).Conclusions BDNF promotes cell proliferation and clone formation of HCAEC,the PI3 K/Akt/mTOR signaling pathway,ERK signaling pathway and Wnt/β-catenin signaling pathway may be involved in.
作者
孔继昌
王恩艳
张巍
戴坤鹏
刘少云
付永杰
郭志强
KONG Ji-chang;WANG En-yan;DAI Kun-peng;GUO Zhi-qiang;LIU Shao-yun;ZHANG Wei;FU Yong-jie(Department of Critical Medicine,General Hospital of North China Petroleum Administration Bureau,Renqiu 062552,China;Department of Internal Medicine‑Cardiovascular,General Hospital of North China Petroleum Administration Bureau,Renqiu 062552,China;Department of Orthopedics,Renqiu People’s Hospital,Renqiu 062552,China;Department of Obstetrics and Gynecology,Renqiu People’s Hospital,Renqiu 062552,China)
出处
《实用老年医学》
CAS
2020年第5期430-433,共4页
Practical Geriatrics
关键词
脑源性神经营养因子
动脉粥样硬化
人冠状动脉内皮细胞
细胞增殖
brain-derived neurotrophic factor
atherosclerosis
human coronary artery endothelial cells
cell proliferation
