摘要
目的:制备一种地龙纳米蛋白复合物材料以解决地龙中促创伤愈合有效蛋白成分容易降解的消耗量过高以及难于运输的问题。方法:以铁镍卤化物为原料,通过高温高压的方法制备得到两种金属氧化物的微球纳米材料,再以自组装的方式装载了地龙蛋白,得到两种纳米地龙蛋白复合物,并进行了药物缓释和创伤愈合测试。结果:合成的纳米材料可以有效维持缓冲液中的蛋白浓度,其中四氧化三铁纳米微球材料可以维持蛋白浓度在0.03mg/mL以上长达约17h。组装后得到两种纳米蛋白复合物(FDPN和NDPN)的小鼠创伤愈合实验结果均证明了复合物在急性炎症期能够有效降低小鼠死亡率,其中FDPN的效果更好。并且FDPN还能较好的促进受损大脑组织的修复。所制备的纳米蛋白复合物在创伤愈合的过程中可以明显促进细胞增殖分裂,加速胶原蛋白合成填充,加速细胞组织的恢复。结论:地龙蛋白纳米材料能够有效的促进创伤愈合,为蛋白类中药材的应用提供了新的思路。
Objective:To solve the problems such as the effective protein components in earthworm that promote wound healing are easily degraded and the consumption is too high and it is difficult to transport by making earthworm nano protein composite material.Methods:Two metal nanoparticles were designed and synthesized as carries to protect and control the releasing of them.The nanomaterials consist of Fe3O4 and NiO individually and were characterized by XRD and SEM.Results:The synthesized nano material could effectively maintain the protein concentration in the buffer solution,where in the ferroferric oxide nano microsphere material could maintain the protein concentration above 0.03mg/mL for about 17 hours.The experimental results of wound healing of mice with two kinds of nano-protein complexes(FDPN and NDPN)obtained after assembly all proved that the complexes can effectively reduce the mortality rate of mice during acute inflammation,and FDPN had better effect.And FDPN could also better promote the repair of damaged brain tissue.The prepared nano protein complex could obviously promote cell proliferation and division,accelerate collagen synthesis and filling,and accelerate cell tissue recovery in the process of wound healing.Conclusion:Earthworm protein nanomaterials can effectively promote wound healing,which gives a new application for inorganic materials in protein drug field.
作者
王东
王雪静
王瑞辉
杨欢
王心怡
唐志书
WANG Dong;WANG Xue-jing;WANG Rui-hui;YANG Huan;WANG Xin-yi;TANG Zhi-shu(Membrane Protein Engineering Laboratory,Medical Scientific Research Center,Shaanxi University of Traditional Chinese Medicine,Xianyang 712046,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第4期2027-2030,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81674088)
国家级大学生创新训练项目(No.201810716015)
陕西省高校科协青年人才托举计划(No.20180308)
陕西省自然科学基金青年项目(No.2018JQ8070)
陕西省教育厅科学研究计划项目(No.18JK0219)
咸阳市科技计划项目(No.2017k02-95)。
关键词
金属氧化物
纳米材料
地龙蛋白
创伤修复
Metal oxides
Nanomaterials
Earthworm proteins
Repair in trauma
