摘要
目的了解厦门市新发现人类免疫缺陷病毒-1(HIV-1)感染者的血浆HIV病毒载量及外周血T淋巴细胞基线情况,探讨病毒载量与外周血T淋巴细胞数量的相关性。方法采用罗氏COBAS TaqMan 48病毒载量仪和贝克曼FC500流式细胞仪检测269例新确证HIV-1感染者的血浆HIV病毒载量和外周血T淋巴细胞,应用SPSS 20软件对检测数据进行分析。结果93.69%(252/269)样本的病毒载量≥103拷贝/mL,病毒载量检测结果为23~1.76×10^6拷贝/mL,对数平均值为(4.43±0.79)log/mL;不同性别、年龄、婚姻状况、文化程度、传播途径的新发现HIV-1感染者的病毒载量水平构成情况比较,差别无统计学意义(P>0.05);血浆病毒载量对数值与CD3^+T淋巴细胞总数、CD8^+细胞计数无显著相关性,但与CD4^+细胞数量和CD4^+/CD8^+比值总体呈负相关[r分别为-0.276(P<0.01)和-0.355(P<0.01)]。结论厦门市大部分新发现的HIV-1感染者体内HIV病毒复制活跃,应考虑联合应用病毒载量和外周血T淋巴细胞基线检测结果指导抗病毒治疗和观测抗病毒治疗效果。
Objective To investigate the base data of plasma HIV viral load and peripheral blood T-lymphocytes in newly detected HIV-1 infected individuals in Xiamen,and discuss the correlation between viral load(VL)and the number of peripheral blood T-lymphocytes.Methods The plasma HIV viral load and peripheral blood T-lymphocytes of 269 newly confirmed HIV-1 infected patients were detected by Roche COBAS TaqMan 48 and Beckman FC500 flow cytometer.The test data were analyzed by using SPSS 20.Results Patients with HIV viral load≥103 copies/mL accounted for 93.69%(252/269).The minimum VL was 23 copies/mL,and the maximum VL was 1.76×10^6 copies/mL,with a logarithmic average of(4.43±0.79)log/mL.Different gender,age,marital status,education level,and transmission route had no statistical significance on the viral load level of newly discovered HIV-1 infected individuals(P>0.05).The plasma viral load logarithm was not significantly correlated with CD3^+T lymphocytes and CD8^+cell count,but negatively correlated with the number of CD4^+cells and the ratio of CD4^+/CD8^+,and the corresponding coefficients were-0.276(P<0.01)and-0.355(P<0.01),respectively.Conclusion In Xiamen,the replication of HIV-1 virus is active in most newly detected HIV-1 infected individuals,so the combination of viral load and peripheral blood T-lymphocyte baseline test results should be considered to guide antiretroviral therapy and observe the effect of antiretroviral therapy.
作者
李莉
张荣秋
马桂林
欧阳雪
LI Li;ZHANG Rongqiu;MA Guilin;OUYANG Xue(Xiamen Center for Disease Control and Prevention,Xiamen 361021,China)
出处
《福建医科大学学报》
2020年第2期113-116,共4页
Journal of Fujian Medical University
基金
福建省科技计划引导性项目(2017D0019)。
