摘要
目的探讨槲皮素(Que)保护全氟辛烷磺酸(PFOS)所致小鼠肝毒性损伤的作用及机理。方法将24只小鼠随机分为4组:对照组、PFOS组、Que组和PFOS+Que组,每组6只,分别给予DMSO、10 mg·kg^-1·d^-1 PFOS、75 mg·kg^-1·d^-1 Que、10 mg·kg^-1·d^-1 PFOS+75 mg·kg^-1·d^-1 Que溶液0.1 mL灌胃,连续给药21 d。比较4组血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平及肝组织丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、C-反应蛋白(CRP)、白介素-6(IL-6)含量。结果与对照组比较,PFOS组血清AST、ALT水平及肝组织MDA、CRP、IL-6含量均显著升高(P<0.05),肝组织SOD、GSH含量均显著降低(P<0.05)。与PFOS组比较,PFOS+Que组血清AST、ALT水平及肝组织MDA、CRP、IL-6含量均显著降低(P<0.05),肝组织SOD、GSH含量均显著升高(P<0.05)。结论 Que能够通过抑制氧化应激和炎症反应保护PFOS所致的小鼠肝损伤。
Objective To investigate the protective effect of quercetin(Que) on perfluorooctane sulfonate(PFOS)-induced hepatotoxicity in the mouse.Methods Twenty-four mice were randomly divided into 4 groups of 6 animals in each:control group(DMSO),PFOS group(10 mg·kg^-1·d^-1),Que group(75 mg·kg^-1·d^-1) and PFOS+Que group(10 mg·kg^-1·d^-1 PFOS+75 mg·kg^-1·d^-1 Que).All animals received intragastric administration(0.1 mL) once daily for 21 consecutive days.Serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activities and hepatic malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH),interleukin-6(IL-6) and C-reactive protein(CRP) contents were compared among the four groups.Results Compared with control group,PFOS treatment increased AST and ALT levels,enhanced MDA,CRP and IL-6 production,and decreased SOD and GSH activities(P<0.05).Compared with PFOS group,serum levels of ALT and AST and hepatic contents of MDA,CRP and IL-6 reduced and hepatic activities of SOD and GSH increased in PFOS+Que group(P<0.05).ConclusionQue can protect against PFOS-induced liver injury in mice by inhibiting oxidative stress and inflammatory response.
作者
林婷婷
黄桃
张文娟
邹惟莹
杨蓓
吴磊
张大雷
LIN Ting-ting;HUANG Tao;ZHANG Wen-juan;ZOU Wei-ying;YANG Bei;WU Lei;ZHANG Da-lei(Department of Physiology,Nanchang University School of Basic Medical Sciences,Nanchang 330006,China)
出处
《南昌大学学报（医学版）》
CAS
2020年第2期45-48,共4页
Journal of Nanchang University：Medical Sciences
基金
国家自然科学基金(81860112)。
关键词
槲皮素
全氟辛烷磺酸
肝毒性
保护
氧化应激
炎症反应
动物
实验
小鼠
quercetin
perfluorooctane sulfonate
protects
hepatotoxicity
oxidative stress
inflammation
animals,laboratories
mice
