摘要
本文通过对1株特殊生境荒漠植物雾冰藜内生真菌Stagonospora sp.的次级代谢产物进行研究,首次分离得到4个松萝酸结构类似物,包括1个新化合物stagonone(1)和3个已知化合物:松萝酸(2),cercosporamide(3)和usnic acid amide(4)。通过高分辨质谱和NMR实验解析了新化合物1的平面结构,采用圆二色谱(CD)方法确定了其绝对构型;本文还报道了松萝酸(2)的单晶结构以及松萝酸(2)的CD谱,为确定该同类化合物的绝对构型提供了支持;活性试验结果证实化合物1-4具有选择性抗肿瘤细胞活性。基于化合物1-4的结构特征,推测了其可能的生物合成途径。
Endophytic fungi from desert plants are a member of specially bioenvironmental microbe with poorly chemical investigated, being one of new resources of bioactive natural products. Chemical investigation of an endophytic fungus Stagonospora sp. inhabiting in desert plant Bassia dasyphylla led to isolate four usnic acid analogs including a new compound stagonone(1) and three known ones, usnic acid(2), cercosporamide(3) and usnic acid amide(4). The structure of the new compound was determined by HRESI-MS, NMR, and circular dichroism(CD) experiments. The X-ray diffraction and CD data of 2, and the cytotoxic activities of 1-4 were also determined. In addition, according to the structural features of 1-4, the possible biosynthetic pathway was postulated.
作者
徐振鲁
王彦多
孙炳达
牛树彬
张永刚
丁刚
XU Zhen-Lu;WANG Yan-Duo;SUN Bing-Da;NIU Shu-Bin;ZHANG Yong-Gang;DING Gang(College of Life Sciences,Shandong Normal University,Jinan,Shandong 250014,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan,Shandong 250103,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;School of Biological Medicine,Beijing City University,Beijing 100083,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第3期581-588,共8页
Mycosystema
基金
中国医学科学院医学与健康科技创新工程协同创新团队项目(2017-I2M-4-004)
国家自然科学基金(31570340和31670011)
天然药物生物活性物质和功能国家重点实验室开放基金(GTZK201903)。
