摘要
本文采用FA-1型六级撞击式空气微生物采样器对上海市某区4个功能区的细菌进行了收集,并应用菌落计数器和显微镜对收集到的细菌进行平皿菌落计数以分析空气中细菌总数分布现状.细菌致病性分析采用崂应2050型空气智能中流量采样器对4个功能区的细菌进行了收集,应用环介导等温扩增技术对特定致病细菌进行检测,并对该区的细菌污染状况进行评价.细菌总数和致病细菌检测结果显示:4个监测站点微生物总量的变化范围是294~1862CFU/m^3,低于3000CFU/m^3,依据《大气微生物评价分级标准》评价,该区的空气始终处于清洁水平,空气质量较好;夏季空气中检出沙门氏菌和金黄色葡萄球菌的概率比秋季高.
The FA-1 type six-stage impactor was used to collect bacteria in four areas of a large coastal city in China.The collected bacteria were counted to analyze the current status of bacterial distribution in ambient air by colony counters and a microscope.The pathogenic bacteria in four functional areas were collected by the Lao Ying 2050 air intelligent medium flow sampler and detected by the loop-mediated isothermal amplification technology.Moreover,the bacterial pollution status of the area was evaluated.The results of total bacteria and pathogenic bacteria have showed that the range of microbial diversity in the four monitoring sites was 294—1862CFU/m^3,which was lower than 3000CFU/m^3.According to classification standard for evaluation of atmospheric microorganisms,the ambient air in this region is always at a clean level with a good quality.And the detecting probability of Salmonella and Staphylococcus aureus occured in summer air is higher than in autumn.
作者
储燕萍
赵望
刘思秀
CHU Yanping;ZHAO Wang;LIU Sixiu(Shanghai Pudong New Area Environmental Monitoring Station, Shanghai 200135, China;Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China)
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2020年第2期240-244,共5页
Journal of Fudan University：Natural Science
基金
上海市浦东新区科委科研项目(PKJ2016-C05)
上海市科委科技创新行动计划(STCSM16441906500)。
关键词
细菌
总数
致病性
环介导等温扩增
bacteria
total number
pathogenicity
the loop-mediated isothermal amplification technology
