摘要
通过40 d的体表暴露试验,研究了饲料中添加α-硫辛酸、抗坏血酸(VC)对鲫鱼暴露于铜绿微囊藻污染水体的保护作用。试验期间水体中微囊藻毒素-LR(MC-LR)质量浓度维持在19.40μg/L左右,试验组饲料中α-硫辛酸和VC的添加量均为600 mg/kg,测定鲫鱼生长性能、MC-LR解毒效果和肝脏抗氧化能力。结果表明,与负对照组相比,饲料中添加α-硫辛酸组和VC组鲫鱼增质量率显著提高(P<0.05),分别提高了4.91%和3.21%;鲫鱼肌肉中MC-LR含量显著降低(P<0.05),分别降低了40.72%、14.20%;肝脏谷胱甘肽过氧化物酶(GSH-Px)活性、超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)显著提高(P<0.05),分别提高了79.35%和72.53%、79.35%和72.53%、34.93%和24.43%;肝脏丙二醛(MDA)含量显著降低(P<0.05),分别降低了57.26%和48.86%。可见,饲料中添加α-硫辛酸、VC能够显著提高鱼体的抗氧化能力,减轻MC-LR对鱼体的氧化损伤,进而减少MC-LR在鱼体内的积累,减轻MC-LR对鲫鱼的毒害作用。
In this study,the effects of dietaryα-lipoic acid and ascorbic acid(VC)on microcystin-induced toxicosis in crucian were investigated by 40-day’s exposure test.During the test,the mass concentration of microcystin-LR(MC-LR)in water was maintained at about 19.40μg/L.The additions ofα-lipoic acid and VC in the experimental group were 600 mg/kg,and the growth performance,MC-LR detoxification and liver antioxidant capacity of the crucian were investigated.Compared with the negative control group,the weight gain rate increased by 4.91%and 3.21%in crucian fed withα-lipoic acid and ascorbic acid(P<0.05),meanwhile,the MC-LR content in the muscle decreased by 40.72%and 14.20%(P<0.05),respectively.Furthermore,activity of glutathione peroxidase(GSH-Px)increased by 79.35%and 72.53%(P<0.05),the activity of superoxide dismutase(SOD)increased by 79.35%and 72.53%(P<0.05),and the total antioxidant capacity(T-AOC)increased by 34.93%and 24.43%(P<0.05),malondialdehyde(MDA)content decreased by 57.26%and 48.86%(P<0.05)in crucian fed withα-lipoic acid and VC.In conclusion,dietaryα-lipoic acid and VC can effectively improve the antioxidant capacity of crucian,reduce the oxidative damage and the accumulation of MC-LR.
作者
徐文杰
韩士群
周庆
巫佳杰
陈婷
XU Wen-jie;HAN Shi-qun;ZHOU Qing;WU Jia-jie;CHEN Ting(Key Laboratory of Agro-Environment in Downstream of Yangtze Plain,Ministry of Agriculture,Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《江苏农业学报》
CSCD
北大核心
2020年第2期417-422,共6页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业科技自主创新基金项目[CX(18)2027]
国家水专项课题(2017ZX07203001)。
