摘要
目的通过改良两种时段接收淋巴结的固定方式,提高淋巴瘤诊断的制片质量。方法随机收集上午10时左右及下午4时左右接收的132例淋巴结标本,每例各切取2块组织,上午切取的组织随机分为2组,分别行短时间固定处理程序(上午短时间组)和延长固定时间处理程序(上午长时间组)进行固定,下午切取的组织也随机分为2组,也分别行短时间固定处理程序(下午短时间组)和延长固定时间处理程序(下午长时间组)进行固定。回顾性分析并筛选出病理诊断为弥漫大B细胞淋巴瘤的58例,其中包括上午10时左右接收的26例及下午4时左右接收的32例。脱水处理后的4组组织进行包埋后,按常规进行切片、HE染色、CD79a免疫组织化学检测、C-MYC双色分离荧光原位杂交实验(fluorescence in situ hybridization, FISH)。通过比较4组切片出现肉眼可见裂隙、皱折及破碎不全发生率、HE染色质量、免疫组织化学检测阳性率、荧光原位杂交成功率,探讨最佳的淋巴结固定方法。结果上午长时间组制片CD79a免疫组织化学检测阳性率、荧光原位杂交成功率均不如上午短时间组,切片不完整发生率及HE染色质量与上午短时间组无明显差异;下午短时间组制片切片不完整发生率高且HE染色质量、CD79a免疫组织化学检测阳性率、荧光原位杂交成功率明显不如上午短时间组;下午长时间组切片以上指标均与上午短时间组无明显差异。结论上午接收的淋巴结标本应剖开于当天进行隔夜常规脱水,下午接收的淋巴结标本必须剖开后行延长固定程序进行脱水,随后得到的淋巴结组织在切片完整性、HE染色、免疫组织化学检测及FISH检测中能更好地提高淋巴瘤诊断的制片质量。
Objective To improve the the slice quality of lymphoma tissue preparation for diagnosis by optimizing the fixation method of lymph nodes received in 2 time frames. Methods 132 lymph node specimens were randomly collected at about 10 a.m. and 4 p.m., and 2 pieces of each specimen were cut from each case. The tissues cut in the morning were randomly divided into 2 groups, which were fixed by short-time fixation treatment program(short-time group in the morning) and extended fixation treatment program(long-time group in the morning), respectively. The tissues cut in the afternoon were also randomly divided into 2 groups, which were also fixed by short-time fixation treatment program(short-time group in the afternoon) and extended fixation treatment program(long-time group in the afternoon). 58 cases of diffuse large B-cell lymphoma diagnosed by pathology were analyzed retrospectively, including 26 cases received at about 10 a.m. and 32 cases received at about 4 p.m. After dehydration and subsequent embedding of tissues in 4 groups, slicing, HE staining, CD79 a immunohistochemical detection(IHC) and C-MYC two-color separation fluorescence in situ hybridization(FISH) were performed. By comparing the incidences of visible cracks, wrinkles and breakage, HE staining quality, positive rate of immunohistochemical detection, and success rate of fluorescence in situ hybridization in 4 groups of sections, the optimal method of lymph node fixation was obtained. Results The positive rate of CD79 a immunohistochemical detection and the success rate of fluorescence in situ hybridization in the morning long-time group were lower than those in the morning shorttime group, while the incidence of incomplete sections and the quality of HE staining were not significantly different in the 2 groups. The incidence of incomplete sections of the afternoon short-time group were higher while the quality of HE staining, the positive rate of CD79 a immunohistochemical detection and the success rate of fluorescence in situ hybridization were sig
作者
张翠霞
方庆全
陈世东
Zhang Cuixia;Fang Qingquan;Chen Shidong(Department of Pathology,2 Department of Clinical Laboratory Medicine,The First Affiliated Hospital of Xiamen University,Xiamen 361003,China;不详)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2020年第1期67-73,共7页
Chinese Journal of Histochemistry and Cytochemistry
关键词
改良
淋巴结固定
淋巴瘤
制片质量
Improved
lymph node fixation
lymphoma
slice quality
