摘要
通过弓形虫RH株速殖子在宿主体内和体外的感染试验,比较了莫能菌素钠、磺胺嘧啶和蒿甲醚的抗弓形虫效果。体外试验:用四甲基偶氮唑盐(MTT)法,评价了3种不同浓度的药物对人包皮成纤维细胞(HFF)毒性及增殖情况;在药物的安全浓度范围内,用5×10^4个弓形虫RH株速殖子感染5×10^5个HFF细胞,每种药物设置5个浓度梯度,培养48 h后观察药物体外抑制虫体在细胞内的增殖效果;以相对抑制率为指标,评估药物的体外抗弓形虫效果。体内试验:用5×10^4个弓形虫RH株速殖子接种小鼠,4 h后,3种药物分高中低剂量组进行灌胃给药,连续给药5 d后停药,观察并记录小鼠每天死亡情况;且每天每组随机抽取1只小鼠,检查腹腔液中速殖子量及停药后是否复发,评价药物体内抗弓形虫效果。体外试验结果表明:莫能菌素钠可显著抑制细胞内弓形虫的增殖,效果优于蒿甲醚,而磺胺嘧啶的效果波动性较大。体内试验结果表明:莫能菌素钠显著延长小鼠平均存活时间,磺胺嘧啶次之,蒿甲醚最次。体内和体外试验结果均显示出莫能菌素钠能显著抑制弓形虫速殖子增殖,延长小鼠存活时间。本研究为后续抗弓形虫药物研究以及临床用药提供了参考依据。
This study evaluated the anti-Toxoplasma gondii effects of monensin natrium,sulfadiazine and artemether through in vivo and in vitro infection with tachyzoite of T.gondii RH strain.In vitro experiment:Toxicity and proliferation of human foreskin fibroblasts(HFF)were evaluated at three different drug concentrations by MTT assay;within the safe concentration range of the drugs,tachyzoites(5×10^4)of T.gondii RH strain were used for HFF cell(5×10^5)infection.The T.gondii in the infected HFF cells were incubated at 5 different concentrations of the drug for 48 h to observe the effect of the drug regarding T.gondii proliferation inhibition in vitro.The relative inhibition rate was used for evaluating the in vitro anti-T.gondii effect of the drug.In vivo experiments:mice were infected with 5×10^4 tachyzoites of the RH strain.Four hours post infection,the mice were treated with the drugs for 5 days at three concentrations(high,medium and low doses)through gavage method.Survival rate of mice was recorded daily.In addition,the amount of tachyzoites in peritoneal fluid was counted daily,aim to check whether the parasite recurred during withdrawal period,and evaluate the in vivo anti-T.gondii effects of the three drugs.The in vitro experiments showed that monensin natrium significantly inhibited the proliferation of T.gondii in cells,and the inhibition effect is better than artemether.The in vivo experiments showed that monensin natrium significantly increased the survival time of infected mice,followed by sulfadiazine and artemether.Both in vivo and in vitro experiments showed that monensin natrium can significantly inhibit the proliferation of T.gondii tachyzoites and prolong the survival time of mice.In summary,this study provides basic references for anti-toxoplasmosis drug research and clinical drug use.
作者
翟斌涛
马元元
谢世臣
彭俊杰
梁盼红
朱兴全
杨晓野
贺君君
ZHAI Bin-tao;MA Yuan-yuan;XIE Shi-chen;PENG Jun-jie;LIANG Pan-hong;ZHU Xing-quan;YANG Xiao-ye;HE Jun-jun(State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2020年第5期624-630,共7页
Chinese Veterinary Science
基金
国家自然科学基金青年项目(31702225)
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-LVRI-03)。
关键词
弓形虫
速殖子
莫能菌素钠
磺胺嘧啶
蒿甲醚
体内试验
体外试验
Toxoplasma gondii
tachyzoite
monensin natrium
sulfadiazine
artemether
in vivo experiments
in vitro experiments
