摘要
通过对大刍草转录组测序来发掘SSR标记位点,结果发现14 099个SSR位点,出现频率为13.31%。三核苷酸和单核苷酸是主要重复类型,分别占SSR总数的42.12%和25.65%;六核苷酸重复所占比例最小,占0.43%。转录组结果表明,大刍草SSR中共发现38种重复基元,单核苷酸重复基元A/T出现频率最高,占总SSR的21.06%;其次为AG/CT和GCC/GGC,分别占6.759%和5.53%。5次重复的SSR数量最多,有3 927个,占总SSR的27.85%。研究结果为大刍草的SSR分子标记研究、遗传多样性分析、种群遗传结构等提供基础。
Single sequence repeat(SSR) marker sites were identified based on transcriptome sequencing of teosinte(Z.mays. ssp. parviglumis) in this study. The results showed that a total of 14 099 SSR were obtained, and the SSRs occurrence frequency f was 13.31%.Trinucleotides and single nucleotides were the main types of repeats, accounting for 42.12% and 25.65% of the total SSRs, respectively;hexanucleotide repeat was the lowest, which was0.43%.Transcriptome results showed that a total of 38 repeat elements were found in the teosinte SSR, and single nucleotide repeat A/T exhibited the highest frequency, accounting for 21.058% of the total SSRs;followed by AG/CT and GCC/GGC, accounting for 6.76% and 5.53% respectively. The number of 5 repeat SSRs was the most, which was 3 927(27.85%). The results should contribute to researches in SSR marker, genetic diversity and population genetic structure in teosinte.
作者
刘强
赵丽
郭虹霞
张丽光
邓妍
王陆军
王创云
LIU Qiang;ZHAO Li;GUO Hong-xia;ZHANG Li-guang;DENG Yan;WANG Lu-jun;WANG Chuang-yun(College of Bio-engineering,Shanxi University,Taiyuan 030006;Institite of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
出处
《玉米科学》
CAS
CSCD
北大核心
2020年第2期69-75,共7页
Journal of Maize Sciences
基金
山西省重点研发计划项目(201703D221001-2)
山西省农科院农业科技创新研究课题项目(YCX2018404)
山西省重点研发计划项目(201703D221001-4)
山西省农科院生物育种工程项目(17yzgc085)
山西省三晋学者支持计划专项经费资助项目(晋教研[2016]7号)
院士工作站建设补助资金(20171049)。
