摘要
目的建立保健食品中六六六、滴滴涕残留量的毛细管气相色谱分析方法。方法采用DB-1701毛细管色谱柱(30 m×0.25 mm,0.25μm),检测器为电子捕获检测器(ECD),比较分析了2种不同前处理方法对六六六、滴滴涕残留量测定的影响。结果六六六的4种异构体和滴滴涕的4种异构体分离良好,峰面积与浓度在0.02μg/ml^0.06μg/ml内呈良好的线性相关性(r>0.9990),检出限为0.34μg/kg^2.14μg/kg,加标回收率为75.9%~112.4%,相对标准偏差(RSD)为1.1%~4.6%。结论2种前处理方法中丙酮-正己烷依次提取比单独用石油醚提取效果好。该方法操作简单,快速,灵敏度和准确度高,适用于各类保健品中六六六、滴滴涕残留量的检测。
Objective To develop a method of capillary gas chromatography for the determination of HCHs and DDTs residues in health food.Methods The analytes were separated with capillary chromatographic column of DB-1701(30 m×0.25 mm,0.25μm)and electron capture detector(ECD);the effects of two different pretreatment methods on the determination of HCHs and DDTs residues were compared and analyzed.Results The HCHs and DDTs were separated,the peak area and the concentration shows good linearity(r>0.9990)within 0.02μg/ml-0.06μg/ml,the detection limits ranged from 0.34μg/kg to 2.14μg/kg,the recovery rates were wihtin 75.9%-112.4%,the RSD was within 1.1%-4.6%.Conclusion In the two pretreatment methods,sequential extraction with acetone-n-hexane is better than extraction with petroleum ether alone.The method is simple,fast,sensitive and accurate,and is suitable for the detection of HCHs and DDT residues in various health products.
作者
张舟艺
张国梅
徐水祥
孙丽华
ZHANG Zhou-yi;ZHANG Guo-mei;XU Shui-xiang;SUN Li-hua(Zhejiang Academy of Medical Science,Health Food Research Institute,Hangzhou,Zhejiang 310013,China)
出处
《中国卫生检验杂志》
CAS
2020年第7期772-774,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省医学支撑学科营养学(11-zc03)
浙江省科技计划项目(2017F30001,2017C25036)
浙江省自然科学基金(LQ18H260003,LQ14H260002)。
