摘要
探讨miR-924影响胃癌MGC803细胞增殖的分子机制。首先预测靶向结合组织型转谷氨酰胺酶(TGM2)的miRNAs,分析miR-924调控TGM2基因3′-UTR结合位点及两者的靶向结合。然后将miR-924导入MGC803细胞,检测其对TGM2表达及MGC803细胞增殖的影响,以及JAK3、STAT3及其磷酸化蛋白表达情况。发现miR-924与TGM2基因3′UTR的864-871位核苷酸靶向结合,并抑制MGC803细胞中TGM2 mRNA及蛋白质表达(P<0.05)。miR-924高表达后MGC803细胞的生长速度减慢,克隆形成能力降低;JAK3与STAT3蛋白磷酸化水平下调(P<0.05)。由此得出,miR-924通过靶向抑制TGM2表达,下调JAK3/STAT3通路活化,降低MGC803细胞增殖。
To investigate the molecular mechanism of the effect of miR-924 on the proliferation of gastric cancer MGC803 cells.Firstly,the microRNAs targeting TGM2 gene and the 3′-UTR binding site of TGM2 Gene regulated by miR-924 were predicted and analyzed.Then,the expression of TGM2,effect of miR-924 on the proliferation of MGC803 and expression of JAK3,STAT3 and their phosphorylated proteins were detected in MGC803 cells.Results showed that miR-924 binds to 864-871 nucleotides of TGM2 gene 3′UTR and inhibits the expression of TGM2 gene and protein in MGC803 cells(P<0.05).After high expression of miR-924,the growth rate of MGC803 cells was slowed down and decreased the number and volume of clones of plate cloning and soft agar colony formation.The phosphorylation of JAK3 and STAT3 proteins in MGC803 cells was decreased after the overexpression of miR-924(P<0.05).miR-924 can inhibit the expression of TGM2,down-regulate the activation of JAK3/STAT3 pathway and reduce the proliferation of MGC803 cells.
作者
周湘华
赵其辉
张志伟
ZHOU Xianghua;ZHAO Qihui;ZHANG Zhiwei(Hunan Environment-Biological Polytechnic College,Hengyang 421001,Hunan,China;Cancer Institute of Medical College,Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan Province,University of South China,Hengyang 421001,Hunan,China)
出处
《中南医学科学杂志》
CAS
2020年第3期244-249,共6页
Medical Science Journal of Central South China
基金
湖南省教育厅平台项目(18K076)
南华大学-岳阳市妇幼保健院合作项目(2018KHX43).
