摘要
【目的】研究枣瘿蚊基因遗传变异程度,分析基因多态性。【方法】从枣瘿蚊部分基因组序列中搜索SSR并设计相应引物,挑选引物48对进行有效筛选。枣瘿蚊样本来自新疆阿拉尔市、昆玉市、且末县等地区,以枣瘿蚊DNA为模板进行PCR扩增,筛选特异性微卫星引物;通过琼脂糖凝胶电泳进行定性检测,再以毛细管电泳检测其多态性。【结果】有42对引物能扩增出条带,选择其中20对特异性较好的引物进行多态性分析。观测杂合度(Observed heterozygosity,Ho)、期望杂合度(Expected heterozygosity,He)和多态信息含量(PIC)平均值分别是0.358、0.649和0.635,其中18对引物PIC值>0.5,具有较高的多态性。【结论】开发的微卫星标记多态性较高,可用于枣瘿蚊种群遗传多样性及遗传结构研究。
【Objective】 The goal was to study the genetic variability of Dasineura jujubifolia genes and analyze its genetic polymorphisms.【Method】 In this experiment, SSR was searched from genome sequences of Dasineura jujubifolia and corresponding primers were designed, from which 48 pairs of primers were selected for effective screening. The Dasineura jujubifolia samples came from Aral, Kunyu and Qiemo and they were amplified by PCR using Dasineura jujubifolia DNA as template. Qualitative detection was performed by agarose gel electrophoresis, and the polymorphism was detected by capillary electrophoresis.【Result】The results show that:There were 42 pairs of primers that could amplify the bands, and 20 with good specificity of them were selected for polymorphism analysis. The average values of observed heterozygosity(Ho), expected heterozygosity(He) and polymorphic information content(PIC)were 0.358, 0.694 and 0.635. Among them, the PIC of 18 pairs of primers was more than 0.5, which was highly polymorphic.【Conclusion】SSR that we developed have high polymorphisms, which can be used to study the genetic diversity and genetic structure of Dasineura jujubifolia population.
作者
马光皇
康淑媛
李莉
肖海兵
熊仁次
杨明禄
MA Guanghuang;KANG Shuyuan;LI Li;XIAO Haibing;XIONG Renci;YANG Minglu(College of Plant Science,Tarim University,Aral Xinjiang 843300,China;Southern Xinjiang Key Laboratory of IPM of Tarim University,Aral Xinjiang 843300,China;Scientific Observing and Experimental Station of Crop Pests in Aral,Ministry of Agriculture,P.R China,Aral Xinjiang 843300,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2020年第5期888-894,共7页
Xinjiang Agricultural Sciences
基金
新疆生产建设兵团国际科技合作计划(2017BC004)
中国农业大学-塔里木大学高校联合基金(2019TC159)。
关键词
枣瘿蚊
微卫星(SSR)
多态性
遗传多样性
Dasineura jujubifolia
microsatellties
polymorphism
genetic diversity
