摘要
【目的】研究外源茉莉酸甲酯(MeJA)对芜菁幼苗形态指标及抗氧化酶活性的影响,为茉莉酸参与芜菁抗逆机理提供理论参考。【方法】以温州盘菜和卡玛古两个商业品种的芜菁作为材料,将萌发的芜菁种子培养于添加不同浓度MeJA的1/2MS培养基中,7 d后测定芜菁幼苗的生长指标、渗透调节物质含量、抗氧化酶活性等生理指标。【结果】随着MeJA浓度增加,芜菁幼苗长度及干、鲜重呈降低趋势,叶片中SOD活性先升高后降低,POD、CAT、APX活性逐渐增高,渗透调节物质可溶性糖、可溶性蛋白、脯氨酸含量逐渐增高,MDA、H2O2和O2·-含量逐渐增高,叶绿素含量逐渐降低。【结论】施加外源MeJA增加了芜菁幼苗的抗氧化酶活性,降低了芜菁幼苗的生长指标,抑制了幼苗的生长。
【Objective】 Different concentrations of exogenous methyl jasmonate(MeJA) were added to the growth process of turnip, and the effects of morphological indexes and antioxidant enzyme activities of turnip seedlings were measured, aiming at providing theoretical reference for the study of its stress resistance mechanism. 【Methods】 The growth and physiological indexes of turnip seedlings were measured after adding MeJA of different concentrations in 1/2 MS medium for 7 days. 【Results】 With the increase of MeJA concentrations, the length, dry and fresh weight of turnip seedlings decreased. The activity of SOD increased first and then decreased, the activity of POD, CAT and APX increased gradually, the contents of soluble sugar, soluble protein and proline, MDA, H2O2 and O2·- increased gradually, and the content of chlorophyll decreased gradually. 【Conclusion】 The above results showed that exogenous MeJA increased the antioxidant enzyme activity of turnip seedlings, but decreased the growth index of turnip seedlings and inhibited the growth of turnip seedlings.
作者
贾凯
闫会转
高杰
JIA Kai;YAN Huizhuan;GAO Jie(College of Forestry&Horticulture,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2020年第5期806-813,共8页
Xinjiang Agricultural Sciences
基金
农业部园艺作物生物学与种质创制综合性重点实验室开放课题(IVF201701)
自治区高校科研计划自然科学项目(XJEDU2017S017)
新疆维吾尔自治区园艺学重点学科基金(2016-10758-3)
新疆农业大学研究生科研创新项目(XJAUGRI2017001)。
关键词
茉莉酸甲酯
芜菁
幼苗
抗氧化酶
methyl jasmonate
turnip
seedling
antioxidant enzyme
