摘要
目的观察补肾化痰方对绝经后骨质疏松(PMOP)模型大鼠骨重建平衡的影响。方法选取6月龄SD雌性大鼠30只,按照随机数字表法将其分为假手术组、模型组及中药组,每组10只。假手术组及中药组均以卵巢切除术造模,假手术组仅切除卵巢旁等量脂肪。术后5周开始灌胃,中药组给予补肾化痰方,假手术组和模型组灌服等量生理盐水。12周末大鼠处死后取材,分别采用酶联免疫吸附测定法、蛋白质印迹法及免疫组化法检测血清及骨组织骨形态发生蛋白2(BMP2)、Runt相关转录因子2(RUNX2)、骨保护素(OPG)、核因子κB受体活化因子配体(RANKL)的含量及定位,采用micro CT检测大鼠的骨密度,比较分析各组间各个指标的差异。结果模型组和中药组大鼠骨密度及血清BMP2、RUNX2、OPG、OPG/RANKL含量均低于假手术组,RANKL含量高于假手术组,差异有统计学意义(P<0.05)。中药组大鼠骨密度及血清BMP2、RUNX2、OPG、OPG/RANKL含量均高于模型组,RANKL含量低于模型组,差异有统计学意义(P<0.05)。模型组和中药组大鼠骨组织BMP2、RUNX2、OPG、OPG/RANKL灰度值均低于假手术组,RANKL灰度值高于假手术组,差异有统计学意义(P<0.05)。中药组大鼠骨组织BMP2、RUNX2、OPG、OPG/RANKL灰度值均高于模型组,RANKL灰度值低于模型组,差异有统计学意义(P<0.05)。免疫组化结果显示,BMP2、RUNX2、OPG的阳性表达主要位于骨髓基质细胞胞浆及骨小梁周边成骨细胞中;RANKL的阳性表达位于骨质边缘破骨细胞中,呈棕黄色颗粒。结论补肾化痰方能够通过BMP2/Smad/RUNX2信号通路及OPG/RANKL/RANK信号通路调控骨重建平衡,从而起到治疗PMOP的作用。
Objective To study the effect of Bushen Huatan Decoction on bone remodeling balance in ovariectomized osteoporosis rats.Methods Thirty-six month old female SD rats were divided into three groups:sham operation group,model group and traditional Chinese medicine group according to the random number table method,with 10 rats in each group.In sham operation group and traditional Chinese medicine group,the model was made by ovariectomy.In sham operation group,only the same amount of fat was removed.5 weeks after operation,rats in traditional Chinese medicine group were given Bushen Huatan Decoction,and rats in sham operation group and model group were given the same amount of normal saline.12 weeks after rats were killed,the content and localization of bone morphogenetic protein 2(BMP2),runt related transcription factor 2(RUNX2),osteoprotegerin(OPG),nuclear factor kappa B receptor activating factor ligand(RANKL)in serum and bone tissue were detected by enzyme-linked immunosorbent assay,Western blot and immunohistochemistry respectively.Bone mineral density of rats was measured by micro CT,and the differences of each index between each group were analyzed.Results Bone mineral density and serum BMP2,RUNX2,OPG and OPG/RANKL contents of model group and traditional Chinese medicine group were lower than those of sham operation group,and RANKL content was higher than that of sham operation group,with statistically significant differences(P<0.05).Bone mineral density and serum BMP2,RUNX2,OPG,OPG/RANKL content of the rats in traditional Chinese medicine group were higher than those in model group,and RANKL content was lower than that in model group,with statistically significant difference(P<0.05).BMP2,RUNX2,OPG and OPG/RANKL gray values of bone tissue in model group and traditional Chinese medicine group were lower than those in sham operation group,and the RANKL gray values were higher than those in sham operation group(P<0.05).BMP2,RUNX2,OPG and OPG/RANKL gray values of bone tissue in traditional Chinese medicine group wer
作者
周亚娜
周广文
张麟
张妍
谭张奎
李章青
黄诗怡
熊梦欣
向楠
ZHOU Ya′na;ZHOU Guangwen;ZHANG Lin;ZHANG Yan;TAN Zhangkui;LI Zhangqing;HUANG Shiyi;XIONG Mengxin;XIANG Nan(Department of Oncology,Hubei Provincial Hospital of Traditional Chinese Medicine,Hubei Province,Wuhan430061,China;College of Acupuncture and Bone Injury,Hubei University of Chinese Medicine,Hubei Province,Wuhan430065,China;Department of Osteoarticular Surgery,Wuhan Hospital of Traditional Chinese and Western MedicineWuhan NO.1 Hospital,Hubei Province,Wuhan430061,China;the First Clinical College,Hubei University of Chinese Medicine,Hubei Province,Wuhan430065,China)
出处
《中国医药导报》
CAS
2020年第11期8-11,24,F0004,共6页
China Medical Herald
基金
湖北省自然科学基金青年基金项目(2019CFB232)
湖北省自然科学基金资助项目(2013CFB466)
湖北省卫生和计划生育委员会湖北中医名师向楠工作室(鄂卫生计生通报[2018]15号)。
