摘要
miRNA与恶性肿瘤患者的诊断和预后密切相关,为了考察miRNA-181a在胃癌细胞增殖和迁移中的作用,本研究检测了miRNA-181a在胃癌组织中的表达,并通过对人胃癌细胞系MGC-803转染miR-181a模拟物或抑制剂来考察miR-181a对细胞迁移和增殖的影响。RT-PCR显示,miRNA-181a在胃癌组织中的表达水平显著高于癌旁组织(p<0.05)。伤口愈合实验和Transwell实验显示,转染miR-181a抑制剂或TGF-β受体2(TGFβR2)过表达的pcDNA3.1质粒均可抑制MGC-803细胞的迁移。EdU实验和CCK-8实验显示,转染miR-181a抑制剂或TGFβR2过表达的pcDNA3.1质粒均可抑制MGC-803细胞的增殖。此外,miR-181a抑制剂处理可使TGFβR2蛋白表达明显升高。然而,miR-181a模拟物或抑制剂处理后TGFβR2mRNA水平没有显著变化。总之,本研究表明高表达的miR-181a通过在转录后抑制TGFβR2蛋白表达来促进胃癌细胞的迁移和增殖。miR-181a有望成为胃癌的潜在治疗靶点。
miRNAs are closely related to the diagnosis and prognosis of patients with malignant tumors.In order to investigate the role of miRNA-181 a in the proliferation and migration of gastric cancer cells,this study examined the expression of miRNA-181 a in gastric cancer tissues and investigated the effects of miR-181 a on cell migration and proliferation by transfecting human gastric cancer cell line MGC-803 with miR-181 a mimic or inhibitor.RT-PCR showed that the expression level of miRNA-181 a in gastric cancer tissues was significantly higher than that in adjacent tissues(p<0.05).Wound healing experiments and transwell experiments showed that transfection of miR-181 a inhibitors or pcDNA3.1 plasmid with high expression of TGF-βreceptor 2(TGFβR2)inhibited migration of MGC-803 cells.The EdU assay and the CCK-8 assay showed that transfection of miR-181 a inhibitors or pcDNA3.1 plasmid with high expression of TGFβR2 inhibited the proliferation of MGC-803 cells.In addition,treatment with miR-181 a inhibitors resulted in a significant increase in TGFβR2 protein expression.However,there was no significant change in TGFβR2 m RNA levels after miR-181 a mimic or inhibitor treatment.In conclusion,this study demonstrates that highly expressed miR-181 a promotes migration and proliferation of gastric cancer cells by inhibiting TGFβR2 protein expression after transcription.miR-181 a is expected to be a potential therapeutic target for gastric cancer.
作者
涂龙霞
Tu Longxia(Huaibei Vocational and Technical College,Huaibei,235000)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第2期852-859,共8页
Genomics and Applied Biology
基金
安徽省高校自然科学研究重点项目(KJ2018A0712)资助。
