摘要
本文对紫娟茶的化学成分进行分离纯化及其抗炎、抗氧化活性进行考察。实验采用现代分离纯化方法,从紫娟茶中共分离得到12个化合物,根据波谱学数据进行结构鉴定为:山柰酚(1)、(+)-表儿茶素(2)、(-)-表儿茶素-3-O-没食子酸酯(3)、杨梅素(4)、表没食子儿茶素-3-O-(3″-O-甲基)没食子酸酯(5)、槲皮素(6)、没食子酸(7)、表没食子儿茶素没食子酸酯(8)、3-O-没食子酰基奎宁(9)、小木麻黄素(10)、1,4,6-三-O-没食子酰基-β-D-葡萄糖(11)、咖啡因(12),其中化合物9为首次从紫娟茶中分离得到。化合物11表现出良好的抗氧化活性,化合物1、3、4、6、8~11均具有显著的抗炎活性。
This paper reported the chemical constituents and their anti-inflammatory and antioxidative activity of biological of Zijuan tea.Zijuan tea is known for purple-colored buds,leaves,stems,calices,peduncles and tea infusion.It was bred from an individual Yunnan Daye Camellia sinensis var.assamica in Yunnan province of China.In this study,column chromatography and high-performance liquid chromatography(HPLC) analysis were used for isolation and purification,12 known compounds were isolated.The structures of them were determined by detailed analysis of their NMR spectra and chemical evidences.The known compounds were identified as kaempferol(1),quercetin(2),(-)-epicatechin-3-O-gallate(3),(+)-epicatechin(4),myricetin(5),epigallocatechin-3-O-(3″-O-methyl) gallate(6),gallic acid(7),epigallocatechin gallate(8),3-O-galloyl quinine(9),strictinin(10),1,4,6-tri-O-galloyl-β-D-glucose(11),caffeine(12),which compound 9 were the first reported from Zijuan tea.Among of them,compound 11 showed favorable antioxidant activity,and compounds 1,3,4,6,8-11 all had significant anti-inflammatory activities.
作者
李明超
桂浩鑫
李晓蕾
刘莹
李宇倩
徐天瑞
郝倩
LI Ming-chao;GUI Hao-xin;LI Xiao-lei;LIU Ying;LI Yu-qian;XU Tian-rui;HAO Qian(Key Laboratory of Screening and Utilization of Targeted Drugs,Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Research Center for Analysis and Mersurement,Kunming University of Science and Technology,Kunming 650093,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第3期414-419,共6页
Natural Product Research and Development
基金
国家自然科学基金(31660099)
云南省科技计划(2017FD100)
云南省教育厅科学研究基金(2019Y0039)。
关键词
紫娟茶
化学成分
抗氧化活性
抗炎活性
Zijuan tea
chemical composition
anti-inflammatory activity
antioxidant activity
