摘要
目的探讨三磷酸腺苷结合盒转运体A1(ATP binding cassette subfamily A member 1, ABCA1)基因多态性与血清脂肪酸结合蛋白3(fatty acid binding proteins 3, FABP3)联合检测在评估冠心病患者多支血管病变中的预测价值。方法选取2018年11月~2019年11月期间在北京大学深圳医院心内科住院治疗的237例冠状动脉粥样硬化性心脏病患者作为研究对象,根据冠状动脉造影结果将其分为单支病变组101例和多支病变组136例,采用PCR方法检测患者ABCA1基因相应片段的多态性,采用单因素及多因素logistic回归分析筛选其独立危险因素,通过ROC曲线分析ABCA1基因多态性和FABP3对多支血管病变的预测价值。结果单因素分析发现,多支病变组患者血清FABP3水平高于单支病变组,差异具有统计学意义(P<0.05),而多支病变组患者ABCA1基因rs363717位点G的突变频率低于单支病变组,差异具有统计学意义(P<0.05)。logistic回归分析提示血清FABP3水平及ABCA1基因rs363717位点的A等位基因是冠心病的独立危险因素。结论 ABCA1基因rs363717位点检测联合血清FABP3对冠状动脉多支血管病变具有较高的阳性预测价值,多支血管病变与rs363717位点多态性及血清FABP3水平密切相关。
Objective To explore the predictive value of combined detection of ABCA1 gene polymorphism and serum FABP3 in evaluating multivessel disease in patients with coronary heart disease(CHD). Methods A total of 237 patients with coronary atherosclerotic heart disease who were hospitalized in the Department of Cardiac Surgery, Peking University Shenzhen Hospital from November 2018 to November 2019 were selected as the research subjects. According to the results of coronary angiography, 101 cases were divided into single-vessel disease group and 136 cases were divided into multi-vessel disease group. The PCR method was used to detect the polymorphism of the corresponding fragment of ABCA1 gene in patients, and the independent risk factors were screened by single factor and multifactor logistic regression analysis. The ABCA1 gene polymorphism and FABP3 pairs were analyzed by ROC curve predictive value of multivessel disease. Results Univariate analysis found that serum FABP3 levels in patients with multivessel disease group were significantly higher than those in single-vessel disease group,the difference was statistically significant(P<0.05) and the ABCA1 gene rs363717 polymorphism was significantly different between the two groups of patients, and the results were statistically significant(P <0.05). Logistic regression analysis suggested that serum FABP3 levels and the A allele at the rs363717 locus of the ABCA1 gene were independent risk factors for coronary heart disease. Conclusion Detection of the ABCA1 gene rs363717 locus combined with serum FABP3 has a high positive predictive value for coronary multivessel disease. Multivessel disease is closely related to rs363717 loci polymorphism and serum FABP3 level.
作者
欧阳春
韩振
叶小强
黄磊
OUYANG Chun;HAN Zhen;YE Xiao-qiang;HUANG Lei(Department of Cardiac Surgery,Peking University Shenzhen Hospital,Guangdong Shenzhen 518036,China)
出处
《现代检验医学杂志》
CAS
2020年第2期1-5,11,共6页
Journal of Modern Laboratory Medicine
基金
深圳市科创委项目(JCYJ20170412150405310)
基20170351以糖酵解为基础的心血管疾病诊断与治疗研究。
关键词
冠心病
三磷酸腺苷结合盒转运体A
脂肪酸结合蛋白3
coronary heart disease
ATP binding cassette subfamily A member
fatty acid binding proteins 3
