摘要
以7种F1代黄瓜(Cucumis sativus L.)未授粉子房为试材进行再生植株培养,通过石蜡切片观察、激素含量测定探究胚囊再生植株形成过程及相关生理变化。结果表明:在MS+0.06 mg·L^-1 TDZ培养基中有3个供体黄瓜材料未授粉子房获得再生植株,经流式细胞检测、染色体计数、SSR验证分别为单倍体、双单倍体和同源四倍体。MS+0.04 mg·L^-1 TDZ+1.0 mg·L^-16-BA+0.1 mg·L^-1 NAA+12mg·L^-1 AgNO3培养基中子房产生大量胚状结构,但分化培养后没有获得再生植株。石蜡切片观察到在MS和MS+0.06 mg·L^-1 TDZ培养基中培养5 d和17 d时胚囊已经发育。供体黄瓜子房切片中GA、IAA含量随培养天数增加呈现先降低后升高的趋势,在培养8 d时最低,17 d时GA含量比2 d时更高,IAA含量无明显变化。
Seven kinds of F1 generation cucumber unpollinated ovary were used as test materials for regenerated plant cultivation.Paraffin sections of ovary were observed,and hormone content of ovary slices at different culture stages were measured to explore the formation process and related physiological changes of embryonary sac regenerated plants.The results showed that the regenerated plants were obtained from unpollinated ovary culture of cucumbers with three donor materials under MS+0.06 mg·L^-1 TDZ medium,which were identified as haploid,double haploid and autotetraploid respectively by flow cytometry,chromosome count and SSR.A large number of embryonary like structures were produced in ovary under MS+0.04 mg·L^-1 TDZ+1.0 mg·L^-16-BA+0.1 mg·L^-1 NAA+12 mg·L^-1 AgNO3 medium,but no regenerated plants were obtained after differentiation culture.The embryonary sac under MS and MS+0.06 mg·L^-1 TDZ medium was observed to develop on the 5 th and 17 th day by paraffin sections.Hormone determination suggested that the content of GA and IAA are on the decline to the lowest at the 8 th day and increased subsequently.The GA content in the ovary slices at 17 d was higher than that in the culture for 2 d,but the variation of IAA content is slight.
作者
周霞
张璐
周俊国
邓英
王怡
娄群峰
陈劲枫
ZHOU Xia;ZHANG Lu;ZHOU Junguo;DENG Ying;WANG Yi;LOU Qunfeng;CHEN Jinfeng(State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;College of Horticulture and Landscape,Henan Institute of Science and Technology,Henan 453000,China;Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2020年第3期455-466,共12页
Acta Horticulturae Sinica
基金
江苏省农业重大新品种创制项目(PZCZ201719)
国家重点研发计划项目(2016YFD0101705-5,2016YFD0100204-25)。
关键词
黄瓜
子房培养
胚囊再生植株
cucumber
ovary culture
embryonary sac regenerated plant
