摘要
目的探讨核转录因子Sp1在手术创伤后腹膜组织中的活性改变与腹膜粘连形成关系。方法选取直肠癌手术患者108例,分为正常腹膜组(开腹后第一时间将腹膜组织从切口左侧2.0 cm处锐性切取)和创伤腹膜组(手术后将腹膜组织从切口右侧2.0 cm处锐性切取),每组各54例。比较两组腹膜组织中Sp1活性,观察Sp1抑制性竞争实验、腹膜组织的胶原纤维变化。结果创伤腹膜组手术后30 min、1 h、2 h、3 h、4 h的Sp1活性逐渐升高(P<0.05),均显著高于正常腹膜组(P<0.05)。50倍未标记特异寡核苷酸完全抑制第3泳道,无条带;50倍未标记变异寡核苷酸未抑制第4泳道,较第1泳道、第2泳道有结合的特异性。结论核转录因子Sp1在手术创伤后腹膜组织中的活化会在一定程度上引发腹膜粘连形成。
Objective To discuss the relationship between the change of nuclear transcription factor Sp1 activity in peritoneal tissue and the formation of peritoneal adhesions after surgical trauma.Methods 108 patients with rectal cancer surgery were selected and equally divided into the normal peritoneum group(the peritoneal tissue was incised sharply from the left side of the incision 2.0 cm at the first time after laparotomy)and the traumatic peritoneum group(the peritoneal tissue was incised sharply from the right side of the incision 2.0 cm after operation).The activity of Sp1 in peritoneal tissue was compared between two groups,and the inhibition competition experiment of Sp1 and the change of collagen fiber in peritoneal tissue were observed.Results The activities of Sp1 at 30 min,1 h,2 h,3 h and 4 h after operation in trauma peritoneum group increased gradually(P<0.05),and were significantly higher than that in normal peritoneum group(P<0.05).50 times of unlabeled specific oligonucleotides completely inhibited the third lane without stripe;50 times of unlabeled variant oligonucleotides did not inhibit the fourth lane,which had binding specificity compared with the first lane and the second lane.Conclusions Activation of nuclear transcription factor Sp1 in peritoneum tissue after surgical trauma can lead to formation of peritoneal adhesions to some extent.
作者
陈立堂
陈勇平
CHEN Litang;CHEN Yongping(Department of Emergency,Huidong County People's Hospital,Huizhou 516300,China;Department of General Surgery,Huidong County People's Hospital,Huizhou 516300,China)
出处
《临床医学工程》
2020年第4期423-424,共2页
Clinical Medicine & Engineering
基金
2019年惠州市科技计划(医疗卫生)项目(2019Y333)。
关键词
核转录因子SP1
手术创伤
腹膜组织
活性
腹膜粘连形成
Nuclear transcription factor Sp1
Surgical trauma
Peritoneal tissue
Activity
Formation of peritoneal adhesions
