摘要
采用对照培养法,探究了绿豆芽生长过程中,萘乙酸(NAA)对营养物质维生素C代谢的影响.绿豆芽用80%甲醇提取,采用高效液相色谱分离/紫外检测,建立了分析绿豆芽中NAA残留的方法.采用C18色谱柱分离,流动相为甲醇和磷酸-氢氧化钠缓冲溶液(0.01 mol·L^-1,pH=3.0)(70∶30,V/V)组成,柱温为40℃,紫外检测波长220 nm.结果表明,绿豆在发芽的过程中,用NAA处理在浓度为0.19μg·mL^-1时维生素C含量达到一个峰值.NAA在0.5~30μg·mL^-1范围内的线性关系良好,相关系数(R2)为0.9997,检出限(S/N=3)为0.01μg·mL^-1,加标回收率在100.2%~110.6%之间.该方法简单、快速,可实现对植物生长激素NAA的残留分析.
In this paper,the effects of naphthalene acetic acid(NAA)on vitamin c metabolism of mungbean sprouts were studied by using control culture.After mungbean sprouts were extracted using 80%methanol,the method for the determination of NAA residues in mungbean sprouts was established by high performance liquid chromatography with ultraviolet detection.The NAA was separated with C18 column.The methanol and phosphoric acid-sodium hydroxide buffer solution(0.01 mol·L^-1,pH=3.0)(70∶30,V/V)was used as mobile phase.The column temperature was 40℃,and UV detection wave length was 220 nm.The results showed vitamin c content was the highest when NAA concentration was 0.19μg·mL^-1.The linear relationship of NAA was good in the range of 0.5~30μg·mL^-1,and the correlation coefficient(R2)was 0.9997.The limit of detection of the method was 0.01μg·mL^-1,and the recovery range was between 100.2%~110.6%.The method is simple,rapid and can be used for the determination of NAA residues.
作者
陈功轩
王杏
张洪权
邓樱花
CHEN Gongxuan;WANG Xing;ZHANG Hongquan;DENG Yinghua(Department of Chemistry and Life Science, Hubei University of Education, Wuhan, 430205, China;Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients,Hubei University of Education, Wuhan, 430205, China)
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第2期224-229,共6页
Journal of Central China Normal University：Natural Sciences
基金
湖北省高等学校优秀中青年科技创新团队计划项目(T201718).
关键词
绿豆芽
萘乙酸
维生素C
高效液相色谱
残留
mungbean sprouts
naphthalene acetic acid
vitamin c
HPLC
residues
