摘要
目的研究沉默miR-16对肺结核模型小鼠免疫功能的调控作用及其机制。方法选取CD2F1雌性小鼠40只,分为正常组、模型组、过表达组、沉默组各10只,模型组、过表达组、沉默组建立肺结核模型,建模成功后,过表达组、沉默组小鼠肺部无菌注射10μL miR-16慢病毒悬液。检测肺组织中T淋巴细胞亚群、胸腺指数、肺部炎症因子以及Toll样受体(TLRs)信号通路因子水平。结果过表达组小鼠CD3+、CD4+、CD4+/CD8+、胸腺指数、IL-10水平低于模型组,CD8+、IL-6、TNF-α、TLR2、TLR4、NF-κB水平高于模型组(P<0.05)。沉默组小鼠CD3+、CD4+、CD4+/CD8+、胸腺指数、IL-10水平高于模型组,CD8+、IL-6、TNF-α、TLR2、TLR4、NF-κB水平低于模型组(P<0.05)。沉默组小鼠CD3+、CD4+、CD4+/CD8+、胸腺指数、IL-10水平高于过表达组,CD8+、IL-6、TNF-α、TLR2、TLR4、NF-κB水平低于过表达组(P<0.05)。结论沉默miR-16可能通过作用于TLRs信号通路,抑制通路因子的异常表达,调节机体免疫反应,改善机体免疫障碍而起到调控肺结核小鼠免疫功能的作用。
Objective To study the regulatory effect and mechanism of silencing microRNA-16 on immune function in mice with pulmonary tuberculosis.Methods Forty CD2 F1 female mice were divided into the control group,model group,overexpression group,and silent group(n=10 per group).In the model group,overexpression group,and silent group,the pulmonary tuberculosis model was established.After successful modeling,the overexpression group and silent group were aseptically injected with 10μL microRNA-16 lentivirus suspension.T lymphocyte subsets,thymus index,lung inflammatory factors and toll like receptor(TLRs)signaling pathway factors were detected in lung tissues.Results The levels of CD3+,CD4+,CD4+/CD8+,thymus index and IL-10 in the overexpression group were lower than those in the model group,while the levels of CD8+,IL-6,TNF-α,TLR2,TLR4 and NF-κB in the overexpression group were higher than those in the model group(P<0.05).The levels of CD3+,CD4+,CD4+/CD8+,thymus index and IL-10 in the silence group were higher than those in the model group,while the levels of CD8+,IL-6,TNF-α,TLR2,TLR4 and NF-κB in the silence group were lower than those in the model group(P<0.05).The levels of CD3+,CD4+,CD4+/CD8+,thymus index and IL-10 in the silence group were higher than those in the over expression group,and the levels of CD8+,IL-6,TNF-α,TLR2,TLR4 and NF-κB were lower than those in the over expression group(P<0.05).Conclusions Silencing microRNA-16 may play a role in regulating the immune function in mice with tuberculosis,by acting on TLR signaling pathways and inhibiting abnormal expression of pathway factors,thus regulating the immune response and improving immune disorders.
作者
王琴
付宇
王平
WANG Qin;FU Yu;WANG Ping(Department of Pediatrics,Renmin Hospital,Hubei University of Medicine,Shiyan 442000,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第4期86-91,共6页
Chinese Journal of Comparative Medicine
