摘要
目的探讨黄连素对糖尿病肾病大鼠晚期糖基化终末产物(AGEs)-晚期糖基化终末产物受体(RAGE)信号通路的影响。方法将84只SPF级健康雄性SD大鼠随机分为空白组、模型组、对照组和低、中、高剂量实验组,每组14只;后面5组大鼠用高糖高脂饮食联合链脲佐菌素腹腔注射建立糖尿病肾病模型。模型建立后,模型组和空白组均按10 mL·kg^-1的剂量灌胃给予0.5%羧甲基纤维素钠溶液,qd;对照组按10 mL·kg^-1的剂量灌胃给予20.0 mg·mL^-1二甲双胍混悬液,qd;低、中、高剂量实验组均按10 mL·kg^-1的剂量分别灌胃给予5.0,10.0和20.0 mg·mL^-1黄连素混悬液,qd。6组大鼠均给药8周。比较各组的空腹血糖(FPG)、血清肌酸酐(SCr),以及肾皮质中AGEs和RAGE表达水平。结果治疗后,低、中、高剂量实验组和空白组、模型组、对照组的FPG分别为(18.33±2.14),(15.02±1.27),(11.94±1.06),(5.16±0.72),(23.25±3.11)和(11.73±1.17)mmol·L^-1,SCr分别为(61.18±5.93),(50.46±4.38),(39.73±3.94),(30.74±3.45),(73.82±8.84)和(38.88±3.71)μmol·L^-1,肾皮质中AGEs表达水平分别为(1.62±0.18),(1.47±0.16),(1.33±0.12),(1.00±0),(1.93±0.25)和(1.30±0.11),肾皮质中RAGE表达水平分别为(1.41±0.17),(1.30±0.04),(1.18±0.13),(1.00±0),(1.64±0.23)和(1.19±0.12),模型组的上述指标与低、中、高剂量实验组和对照组比较,差异均有统计学意义(均P<0.05)。结论黄连素能明显降低糖尿病肾病大鼠的血糖,改善肾功能,其作用可能与抑制肾AGEs-RAGE信号通路活性有关。
Objective To analyze the effects of berberine on advanced glycation end products(AGEs)-receptors for advanced glycation end products(RAGE)signaling pathway in diabetic nephropathy rats.Methods 84 SPF health male SD rats were randomly divided into blank group,model group,control group and experimental-L,-M,-H groups with 14 rats per group,and diabetic nephropathy models were established by intraperitoneal injection of high-sugar and high-fat diet combined with streptozotocin in the latter five groups.After the model was established,model and blank groups were given 0.5%sodium carboxymethyl cellulose solution at the dose of 10 mL·kg^-1 by gavage respectively,qd;control group was given 20.0 mg·mL^-1metformin suspension at the dose of 10 m L·kg^-1 by gavage,qd;experimental-L,-M,-H groups were given 5.0,10.0,20.0 mg·m L^-1 berberinesu spension at the dose of 10 m L·kg^-1 by gavage,qd.Six groups were treated for 8 weeks.The fasting blood glucose(FPG),serum creatinine(SCr),expression levels of AGEs,RAGE in renal cortex were compared among six groups.Results After treatment,the main indexes of experimental-L,-M,-H groups and blank group,model group,control group were compared:FPG were(18.33±2.14),(15.02±1.27),(11.94±1.06),(5.16±0.72),(23.25±3.11)and(11.73±1.17)mmol·L^-1,SCr were(61.18±5.93),(50.46±4.38),(39.73±3.94),(30.74±3.45),(73.82±8.84)and(38.88±3.71)μmol·L^-1,expression levels of AGEs in renal cortex were(1.62±0.18),(1.47±0.16),(1.33±0.12),(1.00±0),(1.93±0.25)and(1.30±0.11),expression levels of RAGE in renal cortex were(1.41±0.17),(1.30±0.04),(1.18±0.13),(1.00±0),(1.64±0.23)and(1.19±0.12).The above-mentioned indexes in model group were significantly different from those in experimental-L,-M,-H groups and control group(all P<0.05).Conclusion Berberine can significantly reduce blood glucose and improve renal function in rats with diabetic nephropathy,which may be related to the inhibition of the activity of renal AGEs-RAGE signal pathway.
作者
黄倩
林佩璜
王梅爱
陈慧勤
郑丹丹
黄秋虹
施子禄
HUANG Qian;LIN Pei-huang;WANG Mei-ai;CHEN Hui-qin;ZHENG Dan-dan;HUANG Qiu—hong;SHI Zi-lu(Department of Physiology,School of Basic Medicine,Quanzhou Medical College,Quanzhou 362100,Fujian Province,China;Department of Nephrology,Quanzhou First Hospital of Fujian Medical College,Quanzhou 362000,Fujian Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2020年第6期632-635,共4页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金面上基金资助项目(31471100)。
关键词
黄连素
糖尿病肾病
肾功能
晚期糖基化终末产物-晚期糖基化终末产物受体信号通路
berberine
diabetic nephropathy
renal function
advanced glycation end products-receptors for advanced glycation end products signaling pathway
