摘要
为了探寻优化山豆根DNA提取的方法,本研究以山豆根的叶片为材料,用3种改良的CTAB法提取山豆根DNA,并将提取的DNA经分光光度计和电泳检测,用于cpDNA引物筛选试验,找出最有效的山豆根DNA提取方法。结果显示,高PVP和β-巯基乙醇提取法和样品预处理法可用于提取山豆根基因组DNA。其中,样品预处理法山豆根DNA的效果最好,提取DNA浓度高、质量好,引物筛选结果条带清晰且单一。本研究建立了山豆根高质量的DNA提取方法,为进行分子生物学研究提供支持。
In order to optimize the method of extracting DNA from the root of Sophora tonkinensis,three improved CTAB methods were used to extract the DNA,from the leaves of Sophora tonkinensis.The extracted DNA was detected by spectrophotometer and electrophoresis,and was used in cpDNA primer screening experiment to find out the most effective method for DNA extraction from Sophora tonkinensis.The results showed that high level of PVP andβ-ME extraction method and sample pretreatment extraction method could be used to extract the genomic DNA of Sophora tonkinensis.Among them,the effect of sample pretreatment was the best,with high concentration and quality of extracted DNA and clear and single primer screening results.In this study,a high-quality DNA extraction method for extracting genomic DNA from Sophora tonkinensis was established to provide support for molecular biology research.
作者
占塔鹏
黄舒婷
乔柱
羽健宾
韦莉
何龙飞
肖冬
Chanthaphoone Keovongkod;Huang Shuting;Qiao Zhu;Yu Jianbin;Wei Li;He Longfei;Xiao Dong(College of Agriculture,Guangxi University,Nanning,530004;Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plants,Nanning,530023)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第8期2585-2590,共6页
Molecular Plant Breeding
基金
广西壮族自治区药用植物园广西药用资源保护与遗传改良重点实验室开放性研究项目(201601)
广西自然科学基金(2017GXNSFAA198252
2016GXNSFBA380223)共同资助。
