摘要
目的:观察穴位埋线联合艾灸对溃疡性结肠炎(UC)大鼠体质量、大便性状、出血情况、肠黏膜组织形态及白细胞介素-6(IL-6)表达的影响,探讨艾灸和埋线联合应用对UC大鼠的抗炎修复作用。方法:将SD大鼠随机分为正常组、模型组、艾灸组、埋线组、埋线加艾灸组,每组6只。采用三硝基苯磺酸联合乙醇灌肠法复制UC大鼠模型。穴位选取双侧"天枢""大肠俞""上巨虚"。艾灸组给予温和灸,每次10 min,每日1次,共干预14次。埋线组给予穴位简易埋线,每周1次,共干预2次。埋线加艾灸组干预方法同艾灸组、埋线组,埋线干预于温和灸干预6 h后进行。观察各组大鼠一般情况、体质量、大便性状、出血情况,并进行疾病活动指数(DAI)评估;HE染色法观察结肠黏膜组织形态变化;免疫组织化学法和Western blot法检测结肠黏膜IL-6的表达。结果:与正常组比较,模型组大鼠饮食减少,活动、精神差,易激惹,毛发少泽,便稀或不成形,肛周红肿伴脓血分泌;治疗后3个治疗组一般情况较模型组均有不同程度的改善。与正常组比较,模型组大鼠DAI明显升高(P<0.01);与模型组比较,埋线加艾灸组、埋线组、艾灸组DAI均明显降低(P<0.01,P<0.05);埋线加艾灸组DAI明显低于埋线组、艾灸组(P<0.01)。HE染色结果显示,正常组结肠组织结构完整清晰;模型组结肠黏膜上层缺损严重,有大量炎性细胞浸润;各治疗组大鼠结肠组织损伤均有所改善,有少量炎性细胞浸润,其中埋线加艾灸组改善最明显。与正常组比较,模型组结肠黏膜组织IL-6阳性表达及蛋白表达量明显增高(P<0.01);与模型组比较,埋线加艾灸组、埋线组、艾灸组结肠黏膜组织IL-6阳性表达及蛋白表达量明显降低(P<0.01,P<0.05);且埋线加艾灸组IL-6明显低于埋线组、艾灸组(P<0.01)。结论:埋线联合艾灸能有效降低UC大鼠DAI,改善结肠黏膜的病理损伤,下调促炎因子IL-6的表达,�
Objective To observe the effect of moxibustion and acupoint catgut embedding(ACE) at "Tianshu"(ST25) "Dachangshu"(BL25) and "Shangjuxu"(ST37) on changes of body mass, stool property, histopathological conditions and expression levels of interleukin 6(IL-6) in colonic mucosa of ulcerative colitis(UC) rats, so as to reveal its anti-inflammatory mechanisms underlying improvement of UC. Methods SD rats were randomized into normal, model, moxibustion, ACE and moxibustion+ACE groups(n=6 in each group). The UC model was established by enema of trinitro-benzene-sulfonic acid and ethanol. Moxibustion was applied to bilateral ST25, BL25 and ST37 for 10 min, once daily for 14 days, and ACE applied to the same 3 acupoints, once a week for two weeks. After the treatment, the rats’ general conditions were observed, and the severity of UC was assessed by using disease activity index(DAI) score. Colonic mucosal pathological changes were observed under microscope after hematoxylin eosin(H.E.) stain, and the expression levels of IL-6 in the colonic mucosa tissue detected by using immunohistochemical stain and Western blot, respectively. Results After modeling, the DAI score, and expression level of colonic IL-6 protein detected by immunohistochemistry and Western blot were obviously increased in the model group relevant to the normal group(P<0.01). Following the intervention, the increase of DAI score and IL-6 expression were reversed in moxibustion, ACE and moxibustion+ACE groups(P<0.01, P<0.05). The therapeutic effects of moxibustion+ACE were considerably superior to those of simple ACE and simple moxibustion in down-regulating the levels of DAI score and IL-6 expression(P<0.01). H.E. staining showed severe defect of the colonic mucosal epithelium with infiltration of a large number of inflammatory cells in the model group, which was milder in moxibustion, ACE and moxibustion+ACE groups. Conclusion Moxibustion combined with ACE is able to improve the inflammatory injury of colonic mucosa in UC rats, which may be related with i
作者
于千惠
李丽红
卢雨微
侯珣瑞
李林
王钰嘉
孔婷婷
梁欣
YU Qian-hui;LI Li-hong;LU Yu-wei;HOU Xun-rui;LI Lin;WANG Yu-jia;KONG Ting-ting;LIANG Xin(Clinical Medical College,Guizhou Medical University,Guiyang 550004,China;The Affiliated Hospital of Guizhou Medical University,Guiyang 550004)
出处
《针刺研究》
CAS
CSCD
北大核心
2020年第4期305-309,共5页
Acupuncture Research
基金
贵州省科技厅科技合作计划项目(黔科合LH字[2017]7220号)。
关键词
艾灸
穴位埋线
溃疡性结肠炎
疾病活动指数
结肠黏膜
白细胞介素-6
抗炎作用
Moxibustion
Acupoint catgut embedding
Ulcerative colitis
Disease activity index
Colonic mucosa
Interleukin-6
Anti-inflammatory effect
