摘要
RNA结合蛋白(RNA binding proteins,RBPs)是一类通过其RNA结合结构域与RNA相互作用的蛋白质,在细胞内发挥着非常重要的作用。RBPs参与从RNA代谢(包括RNA的可变剪接、稳定性、翻译)到表观遗传修饰等多种调控途径。已有大量文献报道转录因子、表观遗传修饰和细胞外信号通路参与调控干细胞的多能性维持、分化和体细胞重编程,但对于RBPs在细胞命运转变中作用的研究报道甚少。该文主要综述了RBPs通过调控RNA的可变剪接、mRNA稳定性、翻译水平、microRNA代谢及组蛋白修饰进而调控干细胞多能性维持和体细胞重编程。
RNA binding proteins are a class of proteins that interact with RNA through their RNA binding domains,and play very important roles in a variety of regulatory pathways,from RNA metabolism(including alternative splicing,stabilization of RNA,translation)to epigenetic modification.It has been reported that transcriptional factors,epigenetic modifications and extracellular signaling pathways play the important roles in regulating pluripotency maintenance and differentiation of pluripotent stem cells and somatic cell reprogramming.However,there are few reports on the role of RNA binding proteins in stem cell fate determination.In this review,we summarize the roles of RBPs in regulating pluripotency maintenance of pluripotent stem cells and somatic cell reprogramming through regulating alternative splicing,mRNA stability,translation level,microRNA metabolism and histone modifications.
作者
王秀芹
尹梦然
夏晴
王丽莎
秦宝明
姚红杰
WANG Xiuqin;YIN Mengran;XIA Qing;WANG Lisha;QIN Baoming;YAO Hongjie(Guangzhou Institutes of Biomedicine and Health,Chinese Academy Sciences,Guangzhou 510530,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2020年第1期145-151,共7页
Chinese Journal of Cell Biology
基金
国家重点研发计划(批准号:2016YFA0100400)
广州再生医学与健康广东省实验室重点研发项目(批准号:2018GZR110104007)资助的课题。
关键词
RNA结合蛋白
多能性
多能干细胞
细胞命运转变
RNA binding protein
pluripotency
pluripotent stem cell
cell fate determination
