摘要
以单针藻Monoraphidium sp.QLZ-3为对象,研究了CO2对微藻在核桃壳提取液(walnut shell extracts,WSE)中生长及油脂积累的影响。结果显示,在12%的CO2条件下,微藻在WSE中的生物量产率及油脂产率达到196.85mg/(L·d)和97.52mg/(L·d),分别是对照组的1.33倍和1.57倍。WSE培养下,外源CO2上调了微藻中核酮糖1,5-二磷酸羧化酶基因(ribulose 1,5-bisphosphate carboxylase/oxygenase,rbcL)的表达量,从而促进了CO2的固定。此外,12%的CO2提高了微藻对WSE中多酚的利用,同时上调了乙酰辅酶A羧化酶(acetyl coenzyme A carboxylase,ACCase)和苹果酸酶(malic enzyme,ME)活性,下调了磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)活性。研究表明,CO2可以提高WSE中微藻的生物量产率和油脂产率,降低培养成本,为核桃壳的资源化利用及微藻的工业化生产提供了新的理论基础。
The effect of CO2 on the cell growth and lipid accumulation of Monoraphidium sp.QLZ-3 in walnut shell extracts(WSE)was studied.Experimental results showed that the biomass and lipid productivities of QLZ-3 were 196.85mg/(L·d)and 97.52mg/(L·d)respectively under the presence of 12%CO2,which were enhanced by 1.33-fold and 1.57-fold compared with the control group,respectively.The exogenous CO2 upregulated the expression level of rbcL that was cultivated in WSE,and thus resulted in an increase in the CO2 fixation.In addition,the 12%CO2 increased the utilization of polyphenol,and increased the activities of the ACCase and ME but decreased those of PEPC of QLZ-3 in WSE.The results indicated that exogenous CO2 could enhance the biomass and lipid productivity and reduce the cost of microalgae cultivation in WSE,which provided a new theoretical basis for the utilization of walnut shell and the industrial production of microalgae.
作者
邢海亮
董训赞
韩本勇
耿树香
宁德鲁
马婷
余旭亚
XING Hailiang;DONG Xunzan;HAN Benyong;GENG Shuxiang;NING Delu;MA Ting;YU Xuya(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,Yunnan,China;Yunnan Academy of Forestry and Grassland,Kunming 650051,Yunnan,China;Yunnan Guiao Agricultural Science and Technology Group,Kunming 650100,Yunnan,China)
出处
《化工进展》
EI
CAS
CSCD
北大核心
2020年第4期1575-1582,共8页
Chemical Industry and Engineering Progress
基金
国家自然科学基金(21766012,21666012)
云南省重大专项(2018ZG003)。
