摘要
旨在揭示BMPR-IB基因在寒泊羊种群中的多态性及遗传学规律,探讨将BMPR-IB基因第746位碱基发生的A→G突变(FecB突变)作为分子标记进行绵羊多胎品种选育的科学性。本研究对寒泊羊育种核心群的健康种公羊、繁殖母羊、羔羊共计1267只绵羊个体进行采血,利用PCR-RFLP方法判定个体BMPR-IB基因位点的基因型并进行群体遗传学分析。对繁殖母羊共计980胎次的产羔记录进行统计,分析FecB突变、胎次及产羔季节对胎产羔数性状的影响。统计所设计杂交组合后代共计167只健康羔羊的基因型比例。结果表明,BMPR-IB基因在寒泊羊种群中有BB、B+和++3种基因型,其基因型频率分别为1.97%、73.40%和24.63%,等位基因B和+的频率分别为38.67%、61.33%;BB、B+和++基因型繁殖母羊的平均胎产羔数分别为2.69、1.91、1.57只,目前寒泊羊种群的胎产羔数平均为1.85只;若经过品种选育使寒泊羊个体中增加一个B基因拷贝,胎产羔数预期增加0.44只;父母本杂交组合为B+×++的后代中B+和++基因型的比例为1.11∶1,父母本杂交组合为B+×BB的后代中BB和B+基因型的比例为0.82∶1,均符合孟德尔分离定律;预测经过6个世代的选育,可使寒泊羊种群母羊基本实现胎产羔数2只的育种目标。本研究结果为绵羊育种实践中制定选种和选配方案提供了理论基础。
The aims of this study were to reveal the polymorphism and genetic regularity of the BMPR-IB gene in Hanper sheep population,and to explore the scientificity to use the A→G mutation(FecB mutation)at the 746 th base of the BMPR-IB gene as a molecular marker for breeding sheep prolific breed.In this study,a total of 1267 sheep individuals were selected,blood samples were collected from healthy breeding rams,breeding ewes,and lambs of the Hanper sheep breeding core population.PCR-RFLP method was used to determine the genotypes of BMPR-IB gene locus in sheep population and analyze the population genetics parameters.The statistics of the lambing records of a total of 980 breeding ewes were analyzed,and the effects of FecB mutation,parity and lambing season on litter size were analyzed.Genotype frequency of 167 healthy lambs from the offspring of the designed hybrid combination was counted.The results showed that BMPR-IB gene had BB,B+and++genotypes in Hanper sheep population,the frequencies of the 3 genotypes were 1.97%,73.40%and 24.63%,respectively,the allele frequencies of B and+were 38.67%and 61.33%,respectively.The litter size of breeding ewes with the BB,B+and++genotypes were 2.69,1.91 and 1.57,respectively,the average litter size of the current Hanper sheep population was 1.85;One copy of B allele was added to the individual of Hanper sheep by breeding,the litter size was expected to increase by 0.44.The ratio of B+and++genotypes in the offspring of hybrid combination of B+×++was 1.11∶1,and the ratio of BB and B+genotypes in the offspring of hybrid combination of B+×BB was 0.82∶1,both conforming to Mendelian separation law.It was predicted that after 6 generations of breeding,litter size of Hanper sheep ewes could basically achieved 2 lamds.The results of this study provide a theoretical basis for the development of breeding and selection schemes in sheep breeding practice.
作者
马晓菲
刘爱菊
韩红叶
孙欢
孙克佳
李晓双
孙树春
田树军
MA Xiaofei;LIU Aiju;HAN Hongye;SUN Huan;SUN Kejia;LI Xiaoshuang;SUN Shuchun;TIAN Shujun(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China;Hebei Province Cattle and Sheep Embryo Engineering Technology Research Center,Baoding 071000,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2020年第4期688-700,共13页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
河北省羊产业技术体系(HBCT2018140202)。
