摘要
目的探讨黄芪注射液对db/db小鼠免疫细胞及炎性因子的调节作用及可能的作用机制.方法将C57BL/6小鼠作为对照组,db/db小鼠随机分成糖尿病组和糖尿病黄芪注射液治疗组.对照组和糖尿病组小鼠每日分别腹腔注射PBS 100μL,糖尿病黄芪注射液治疗组小鼠每日腹腔注射黄芪注射液100μL,连续12周.12周后检测外周血白细胞数量及分类;ELISA法检测血清中白细胞介素-10(IL-10)、肿瘤坏死因子α(TNF-α)的表达;实时PCR检测小鼠肾脏白细胞介素-1β(IL-1β)、TNF-α和人单核细胞趋化蛋白-1(MCP-1)的mRNA表达;流式检测脾脏中调节性T细胞(Tregs)细胞百分含量.结果外周血白细胞数、中性粒细胞百分比和单核细胞百分比,糖尿病组明显高于对照组,黄芪注射液治疗组较糖尿病组明显下降.糖尿病组小鼠血清中IL-10显著低于对照组,TNF-α显著高于对照组,黄芪注射液治疗组的IL-10显著高于糖尿病组,而TNF-α则显著低于糖尿病组.糖尿病组IL-1β、MCP-1、TNF-α的mRNA表达显著高于对照组,黄芪注射液治疗组又显著低于糖尿病组;糖尿病组Tregs细胞数量高于对照组,而黄芪注射液治疗组又显著高于对照组.结论黄芪注射液可以通过降低db/db小鼠炎症细胞数量,增加Tregs细胞数量,降低促炎因子IL-1β、MCP-1和TNF-α的表达;延缓糖尿病肾病(DN)的发生.
Objective To investigate the effects and the mechanism of Astragalus injection regulating immune cells and Inflammatory factors in db/db mice.Methods C57BL/6 mice were set as the control group,db/db mice were randomLy divided into diabetic group and astragalus-treated group.The mice of control group and diabetic group were intraperitoneally injected with PBS 100 μL,and the mice of astragalus-treated group were intraperitoneally injected with astragalus mongholicus 100μL,every day for 12 weeks.Twelve weeks later,blood glucose and the counting and classification of leukocytes of the peripheral blood were detected.The expression of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)was detected by ELISA in cell culture supernatant.The mRNA expression of interleukin-1β(IL-1β), TNF-αand monocyte chemoattractant protein-1(MCP-1)in mouse kidney was detected by real-time PCR.The percent of regulatory T cells(Tregs)was detected by flow cetometry.Results Blood glucose in diabetic group is higher than that in control group.There is no significant difference between astragalus-treated group and diabetic group.The counting leukocytes and the percentage of neutrophils and mmonocyte in diabetic group obviously were improved,and that in astragalus-treated group were decreased obviously.IL-10 in the serum in diabetic group was obviously lower than that in control group.However,IL-10 in astragalus-treated group was significantly higher than that in diabetic group. TNF-α in the serum in diabetic group was obviously higher than that in control group,and that in astragalus-treated group was significantly lower.mRNA expression of renal inflammatory factors in mice showed that the mRNA expression of IL-1β,MCP-1 and TNF-α in the diabetes group was significantly higher than that in the control group,and that in the astragalus-treated group was significantly lower than that in the diabetes group.The quantity of Tregs in diabetic group was improved,and that in astragalus-treated group was significantly higher.Conclusion Ast
作者
朱丽坤
曹爽
蒋仕林
尹晓琳
ZHU Likun;CAO Shuang;JIANG Shilin;YIN Xiaolin(Department of Clinical Laboratory,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050017,China)
出处
《实验动物与比较医学》
CAS
2020年第1期60-64,共5页
Laboratory Animal and Comparative Medicine
基金
河北省自然科学基金(H2014206212)。
关键词
黄芪注射液
糖尿病肾病(DN)
炎症反应
炎性因子
Astragalus injection
Diabetic nephropathy
Inflammatory reaction
Inflammatory factor
