摘要
氮是植物生长必须的大量元素之一,大豆根瘤菌共生可以为大豆提供充足的氮元素。研究大豆根瘤菌共生机理,挖掘控制共生结瘤候选基因具有重要意义。鉴于现阶段关于调控大豆共生结瘤QTL区间大,无法直接应用到实际中,本实验整理了68个控制大豆结瘤的QTLs,通过Overview和共线性分析对其进行优化,在Gm06染色体上得到一个高置信QTLs区间对该区间进行了基因注释得到43个基因,其中包括一个控制C2钙/脂质结合和含GRAM结构域的蛋白质,一个侧根形成蛋白和一个E3泛素连接酶相关蛋白,并在CSSLs群体中查找该位置存在插入片段的株系进行结瘤性状鉴定。结果表明C2连锁群上的15-15.5Mb对于大豆根瘤菌共生结瘤有着至关重要的作用。
Nitrogen is one of the many elements necessary for plant growth.Symbiosis of soybean rhizobium can provide sufficient nitrogen for soybean.It is important to study the mechanism of soybean rhizobium symbiosis and excavate candidate genes for controlling symbiosis.Given that the QTL for regulating soybean symbiosis is large at this stage,it cannot be directly applied to practice.In this experiment,68 QTLs for soybean nodulation were col lated,and they were optimized by Overview and colinearity analysis.A high-confidence QTLs interval was obtained on the chromosome Gm06.The gene was annotated and 43 genes were obtained,including one coding system C2 Calcium/lipid binding and GRAM domain-containing proteins,a side root-forming protein and an E3 ubiquitin li gase-related protein,and it’s a so possible to search for strains with inserts at this location in the CSSLs population for identification of nodulation traits.The results showed that 15-15.5 Mb on the C2 linkage group was crucial for the symbiotic nodulation of soybean rhizobium.
作者
李健依
王悦
辛大伟
李瑞超
于晶瑶
黄仕钰
王新宇
武小霞
陈庆山
LI Jian-yi;WANG Yue;XIN Da-wei;LI Rui-chao;YU Jing-yao;HUANG Shi-yu;WANG Xin-yu;WU Xiao-xia;CHEN Qing-shan(Laboratory of Soybean Genetic Improvement,Northeast Agricultural University,Harbin 150030,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2020年第1期37-43,共7页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31771882
31971899)
黑龙江省自然科学基金(C2018017)
吉林省农业科技创新项目(C92070420)
国家重点研发计划子项目(2016YFD0100201-21)
黑龙江省杰出青年自然科学基金(JC2017006
JC2016004)。
