摘要
为了评价抗虫转基因甘蔗优良株系Bt2、Bt17对土壤生态环境造成的生态安全风险,采集抗虫转基因甘蔗Bt2、Bt17号株系及其受体非转基因品种ROC22甘蔗根际附近土壤,研究抗虫转基因甘蔗对其根际土壤蔗糖酶、酸性磷酸酶、中性磷酸酶及碱性磷酸酶的影响。结果表明,抗虫转基因甘蔗对其根际土壤酶活性的影响会因甘蔗生长周期、抗虫转基因甘蔗株系以及酶的种类而大有不同。Bt2的土壤酶活性在甘蔗生长的各个时期均未与对照存在差异,而Bt17对土壤酶活性的影响则较为复杂。与受体非转基因甘蔗品种ROC22相比,Bt17根际的土壤蔗糖酶活性在甘蔗生长的任何时期都无差异;而土壤酸性磷酸酶活性在甘蔗生长的各个时期均显著高于对照;中性磷酸酶活性则在苗期、分蘖期、成熟期显著高出对照,而在生长期与对照无差异;土壤碱性磷酸酶活性在苗期、生长期时与对照无差异,但分蘖期和成熟期显著低于对照,说明Bt2号株系对土壤酶活性并未产生影响,Bt17号株系对土壤酶活性可能产生较小的影响。
In order to assess the ecological risks of insect-resistant transgenic sugarcane lines Bt2 and Bt17 on soil ecological environment rhizosphere soil samples were collected from plantations of insect-resistant transgenic sugarcane lines Bt2 and Bt17 and their non-transgenic donor sugarcane variety ROC22 to analyze the effect of the insect-resistant transgenic sugarcane lines on the activities of soil sucrase,acid phosphatase,neutral phosphatase and alkaline phosphatase in the rhizosphere.The results showed that the effects of the insect-resistant transgenic sugarcane lines on the soil enzyme activities in the rhizosphere varied greatly with the growth cycle of sugarcane,the insect-resistant transgenic sugarcane lines and the enzymes.Soil enzyme activity of sugarcane line Bt2 was not different from that of the control at all the stages of sugarcane growth,while the effect of Bt17 on soil enzyme activity was more complex.Compared with the non-transgenic donor sugarcane ROC22,the sugarcane line Bt17 was not different in soil sucrase activity in the rhizosphere at any stage of sugarcane growth,significantly higher in soil acid phosphatase activity at any stage of sugarcane growth,significantly higher in neutral phosphatase activity at seedling stage,at tillering stage and at mature stage,but not different in neutral phosphatase activity at the grand growth stage,but significantly lower in neutral phosphatase at the tillering stage and the mature stage.This indicated that the sugarcase line Bt2 had no effect on soil enzyme activity in the rhizosphere,while the sugarcane line Bt17 might have less effect on soil enzyme activity in the rhizosphere.
作者
冯翠莲
万玥
赵婷婷
王俊刚
冯小艳
张树珍
FENG Cuilian;WAN Yue;ZHAO Tingting;WANG Jungang;FENG Xiaoyan;ZHANG Shuzhen(Institute of Bioscience and Biotechnology/Sugarcane Research Center/Ministry of Agriculture and Rural Affairs Key Open Laboratory for Bioscience and Biotechnology of Tropical Crops,CATAS,Haikou,Hainan 571101,China;College of Life Science,Nanjing Agriculture University,Nanjing 210095,China)
出处
《热带生物学报》
2020年第1期1-6,共6页
Journal of Tropical Biology
基金
中国热带农业科学院基本科研业务费专项(1630052020005)
海南省青年科技英才创新计划项目(HAST201626)
国家糖料产业技术体系“甘蔗病毒病防控岗位科学家”经费(CARS-170301)。
关键词
抗虫转基因
甘蔗
土壤酶活性
生态安全
insect-resistant transgene
sugarcane
soil enzyme activity
ecological risk
