摘要
[目的]筛选耐高温秸秆降解菌,并对其进行鉴定,分析复合菌剂的秸秆降解效果。[方法]从河南地区农田腐烂的玉米秆、麦秸秆、枯枝败叶中通过分离培养基分离出4株耐高温且具有纤维素降解能力的菌种,并对其进行16/18SrRNA鉴定,NGW11菌株为米曲霉菌,NGW12菌株为芽枝孢霉菌,NGW13菌株为枯草芽孢杆菌,NGW14菌株为苏云金芽孢杆菌,复合菌液降解菌种组合为3︰1︰4︰2。[结果]通过DNS法测定各菌株对不同秸秆的纤维素降解酶活,结果表明复合菌液对不同秸秆都有较好的降解效果。
[Objective]To screen high temperature resistant straw degradation bacteria,identify them and analyze the straw degradation effect of the compound bacteria.[Methods]Four strains with high temperature resistance and cellulose degradation ability were isolated from corn straw,wheat straw and withered leaves in farmland of Henan Province,and identified by 16/18srRNA.NGW11 was Aspergillus oryzae,NGW12 was mycorrhiza,NGW13 was Bacillus subtilis,NGW14 was Bacillus thuringiensis,which was degraded by compound bacterial solution,the combination of strains was 3︰1︰4︰2.[results]the enzyme activity of cellulose degradation of different straws was determined by DNS method,and the results showed that the compound bacterial solution had better degradation effect on different straws.
作者
孙学习
习会丽
轩云梦
何显
汪雪
Sun Xuexi;Xi Huili;Xuan Yunmeng;He Xian;Wang Xue(College of Chemical Engineering and Food,Zhengzhou Institute of Technology,Zhengzhou 450044,China)
出处
《广东化工》
CAS
2020年第5期50-52,共3页
Guangdong Chemical Industry
基金
河南省重点研发与推广专项科技攻关项目(182102310024)
郑州工程技术学院高层次人才基金项目。
关键词
耐高温
秸秆降解菌
复合菌剂
high temperature resistance
straw degradation bacteria
compound bacteria
