摘要
背景:左旋甲状腺素可以显著改善亚临床甲减的症状,也有研究指出左旋甲状腺素可部分改善实验大鼠的骨代谢异常,但其对于亚临床甲减性骨质疏松的治疗作用却鲜有研究。目的:在亚临床甲减大鼠模型上观察有氧运动联合左旋甲状腺素与维生素D3对骨质疏松症状的改善作用。方法:将Wistar大鼠分为空白对照组、假手术组、模型组进行造模,并检测大鼠甲功指标,此为造模阶段;造模成功后将模型组分为无处理组、运动组、左旋甲状腺素组、维生素D3组、运动+左旋甲状腺素组、运动+维生素D3组、左旋甲状腺素+维生素D3组、运动+左旋甲状腺素+维生素D3组,另设置一组正常对照组,其中正常对照组不作任何处理,其余各组分别接受相应的单一因素或者联合因素处理,共52 d,此为处理阶段。随后,检测大鼠血清中骨吸收标志物(β-Ⅰ型胶原羧基端肽和血清抗酒石酸酸性磷酸酶5b)、骨形成标志物(骨型碱性磷酸酶、Ⅰ型前胶原氨基端前肽和血清骨钙素)等骨代谢指标,并对大鼠头骨、脊柱、上肢和下肢进行骨密度扫描,测量各组大鼠血清中钙和磷及右股骨组织中CathepsinK的蛋白水平,对各组大鼠股骨头行苏木精-伊红染色。结果与结论:(1)在造模阶段,相对于空白对照组与假手术组,模型组大鼠血清中促甲状腺素水平显著增加(P<0.05),而血清FT3、FT4无明显变化,说明亚临床甲减大鼠造模成功。(2)进行干预后,与其他无甲状腺素处理的各组大鼠相比,4个补充左旋甲状腺素组大鼠的血清促甲状腺素水平明显降低(P<0.05),而T3、T4无明显变化,但其骨代谢指标和骨密度则显著升高(P<0.05),且以运动+左旋甲状腺素+维生素D3组改善最为显著(P<0.05)。左旋甲状腺素+维生素D3组及运动+左旋甲状腺素+维生素D3组大鼠中的血清钙和磷水平显著高于其他无维生素D3处理组(P<0.05);4个补充左旋甲状腺素组�
BACKGROUND:Levothyroxine can significantly improve the symptoms of subclinical hypothyroidism,and some studies have pointed out that levothyroxine can partially improve the abnormal bone metabolism of experimental rats,but the therapeutic effect of levothyroxine on subclinical hypothyroid osteoporosis is rarely studied.OBJECTIVE:To observe the effects of aerobic exercise combined with levothyroxine and vitamin D3 on the symptoms of osteoporosis in subclinical hypothyroidism rats.METHODS:Wistar rats were divided into blank control group,sham-operated group and model group.The thyroid function was measured to determine whether the model was successfully established.The model group rats were further divided into eight groups:non-treatment group,exercise group,L-thyroxine group,vitamin D3 group,exercise+levothyroxine group,exercise+vitamin D3 group,levothyroxine+vitamin D3 group and exercise+levothyroxine+vitamin D3 group,with another normal control group.At the 52 th day after treatments,the bone resorption markers,βisomer of C-terminal telopeptide of type I collagen(β-CTX)and tartrate-resistant acid phosphatase-5 b(TRACP-5 b),and the bone formation markers,bone-specific alkaline phosphatase(BLAP),type I procollagen amino-terminal propeptide(PINP),and serum osteocalcin(BGP)were detected.Bone mineral density of the rat skull,spine,upper limb and lower limb was scanned.Serum calcium and phosphorus levels and cathepsin K level in the right femur were measured.Hematoxylin-eosin staining of the rat femoral head was performed.RESULTS AND CONCLUSION:At the modeling stage,serum thyroid-stimulating hormone(TSH)level of rats in the model group was significantly higher than that in the sham-operated group and blank control group(P<0.05).But there was no significant difference in serum T3 and T4 among the groups,indicating that the subclinical hypothyroidism rat model was successfully established.After treatment,compared with the rats without levothyroxine treatment,serum TSH levels in the rats of the levothyroxine group,the
作者
温霜威
武青梅
Wen Shuangwei;Wu Qingmei(School of Physical Education,Jinzhong University,Jinzhong 030619,Shanxi Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第26期4118-4124,共7页
Chinese Journal of Tissue Engineering Research
