摘要
目的研究湖州地区诺如病毒(NoV)的流行病学模式和遗传特征。方法2015年1月-2017年12月,收集急性胃肠炎患者的粪便标本,进行实时RT-PCR(qPCR)检测NoV。RT-PCR用于基因组扩增和序列测定。病毒基因型别使用在线NoV分型工具(http://www.rivm.nl/mpf/norovirus/typingtool)分型。结果NoV感染的总体流行率为23.7%(414/1757)。在414个NoV阳性标本中,393个被鉴定为NoV GⅡ,11个属于NoV GⅠ,10个是NoV GⅠ和GⅡ共同感染。共235份标本成功测序,这3年的主要流行型别是GⅡ.Pe-GⅡ.4 Sydney2012和GⅡ.P17-GⅡ.17,2017年还出现新的流行优势株GⅡ.P16-GⅡ.2。全年均检测到NoV感染,流行高峰发生在每年的冬春期间。结论在2014年10月GⅡ.17首次在湖州出现后,稳步取代了以前流行的GⅡ.4 Sydney 2012菌株。但在2016年GⅡ.4 Sydney 2012又回到主导地位,2017年出现新的流行优势株GⅡ.P16-GⅡ.2。
Objective To study the epidemiological patterns and genetic characteristics of norovirus(NoV)in Huzhou area.Methods From January 2015 to December 2017,stool samples from patients with acute gastroenteritis were collected for the detection of NoV by real-time RT-PCR(qPCR).RT-PCR is used for genome amplification and sequencing.Virus genotyping was performed using the online NoV typing tool(http://www.rivm.nl/mpf/norovirus/typingtool).Results The overall prevalence of NoV infection was 23.7%(414/1757).Of 414 NoV positive specimens,393 were identified as NoV GⅡ,11 were NoV GⅠ,and 10 were co-infected with NoV GⅠand GⅡ.A total of 235 specimens were successfully sequenced.The main epidemic types in these three years were GⅡ.Pe-GⅡ.4 Sydney 2012 and GⅡ.P17-GⅡ.17.A new dominant strain GⅡ.P16-GⅡ.2 was also found in 2017.NoV infection was detected throughout the year,and the peak occurred in winter and spring every year.Conclusion After GⅡ.17 first appeared in Huzhou in October 2014,it steadily replaced the previously prevalent GII.4 Sydney 2012 strain.However,in 2016,GⅡ.4 Sydney 2012 returned to the dominant position,and a new predominant strain GⅡ.P16-GⅡ.2 appeared in 2017.
作者
陈莉萍
纪蕾
沈月华
徐德顺
CHEN Li-ping;JI Lei;SHEN Yue-hua;XU De-shun(Huzhou Center for Disease Control and Prevention,Huzhou,Zhejiang 313000,China)
出处
《中国卫生检验杂志》
CAS
2020年第6期666-668,672,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江医药科技计划项目(2018RC015)
湖州市科技局自然科学基金(2017YZ04)。
