摘要
目的采用色谱轮廓及化学计量学结合的方法对不同生产企业生产的柴胡配方颗粒进行差异性研究,以促进柴胡配方颗粒的质量一致性。方法研究建立了柴胡配方颗粒高效液相210和254 nm指纹图谱,并且通过相似度分析、聚类分析、主成分分析以及偏最小二乘判别分析分别对双波长指纹图谱进行了质量差异特征性分析。结果建立了12批柴胡配方颗粒的指纹图谱。结果表明,所建立的双波长指纹图谱可以对不同生产企业生产的柴胡配方颗粒进行区分,也进一步明确了不同生产企业生产的柴胡配方颗粒指纹图谱特征色谱峰的位置。结论柴胡配方颗粒的210 nm指纹图谱的特征差异最大,建议双波长指纹图谱结合一起进行分析及应用;柴胡皂苷a、柴胡皂苷b2和柴胡皂苷b1为质量差异的关键组分,应在对柴胡配方颗粒进行质量评价与控制时重点关注。
OBJECTIVE To distinguishing the Bupleurum formulae granules from different origins and promoting the consistency of quality of them,with the analysis of fingerprint profiles and chemometric methods. METHODS In the study,a method of dualwavelength( 210 & 254 nm) HPLC fingerprint of Bupleurum formulae granules was developed. Based on the fingerprint method,the quality differences of the samples of Bupleurum formulae granules were analyzed by similarity analysis,hierarchical cluster analysis,principal component analysis and partial least squares discriminant analysis. RESULTS The fingerprints of 12 batchs of Bupleurum formulae granules are established. The results indicated that the fingerprints of Bupleurum formulae granules from different origins could be differentiated well and the characteristic peaks could be marked. CONCLUSION The 210 nm fingerprints of Bupleurum formulae granule have the most characteristic differences;It is recommended that the dual-wavelength fingerprints could be combined for analysis and application. Saikosaponin a,saikosaponin b2 and saikosaponin b1 are the key components of the quality difference,which should be focused on in the quality evaluation and control of Bupleurum formula granules.
作者
王晓伟
王艳伟
王海波
刘瑞新
石岩
WANG Xiao-wei;WANG Yan-wei;WANG Hai-bo;LIU Rui-xin;SHI Yan(Henan Provincial Institute of Food and Drug Control,Zhengzhou 450008,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China;National Institutes for Food and Drug Control,Beijing 100050,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2020年第3期218-227,共10页
Chinese Pharmaceutical Journal
基金
国家自然科学基金面上项目资助(81773892)。
关键词
柴胡
配方颗粒
指纹图谱
质量差异
化学计量学
Bupleurum
formulae granule
fingerprint
quality difference
chemometrics
