摘要
该文设计9种分离培养基,采用稀释涂布法从14份真红树植物的46份组织样品中分离纯化内生细菌。并基于菌株形态学特征和16S rRNA基因序列确定分离菌株的种属及分析其物种多样性,采用秀丽隐杆线虫模型筛选菌株延缓衰老活性。结果表明:(1)通过基因序列去重复后从46份真红树植物组织样品中获得32株海洋细菌,基于菌株16S rRNA基因序列信息分析,覆盖12科17属,其中芽孢杆菌属(Bacillus)为优势菌属,并获得1株疑似橙单胞菌属(Aurantimonas)新种,16S rRNA基因序列相似性低于97%;(2)经过秀丽隐杆线虫粗筛发现3株海洋细菌具有显著延缓线虫衰老的活性(P<0.05)。以上结果表明海南西海岸真红树内生细菌具有物种多样性,部分菌株具有延缓线虫衰老活性。
The bacteria were isolated and purified from 46 tissues of 14 true mangrove plants used nine kinds of isolation media by dilution coating method.The bacteria were isolated from strains,and the species and genus diversities were analyzed by bacteria morphological characteristics and 16S rRNA gene sequences.The anti-aging activities of the bacteria were tested by Caenorhabditis elegans screening models.The results were as follows:(1)The 32 marine bacteria were obtained from 46 tissues of true mangrove plants without repeatitions.The 32 bacteria to comparison of 16S rRNA gene sequences which could be classified into 12 families and 17 genera.The dominant genus were Bacillus.It was discovered that a novel strain of suspected genus Aurantimonas,which 16S rRNA gene sequence similarities were less than 97%;(2)The three cultured marine bacteria could significantly delay Caenorhabditis elegans developing and had dominant anti-aging activities(P<0.05).All the above results indicate that the true mangrove plants collected from the west coast of Hainan have high species diversity and part strains are rich in anti-aging activity.
作者
李蜜
高程海
姜舒
韦娟
刘思萍
戴恩枝
易湘茜
LI Mi;GAO Chenghai;JIANG Shu;WEI Juan;LIU Siping;DAI Enzhi;YI Xiangxi(Institutes of Marine Drugs/Faculty of Pharmacy/Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Guangxi University of Chinese Medicine,Nanning 530200,China)
出处
《广西植物》
CAS
CSCD
北大核心
2020年第3期311-319,共9页
Guihaia
基金
国家自然科学基金(41566004)
广西中医药大学岐黄工程高层次人才团队培育项目(2018006)
广西中医药大学2017年引进博士科研启动基金(2017BS039)
广西中药药效研究重点实验室资助项目(17-259-20)。
关键词
真红树植物
内生细菌
物种多样性
延缓衰老活性
true mangrove plants
endophytic actinobacteria
species diversity
anti-aging activity
