摘要
背景:前期研究发现不同管径钛纳米管对植体表面细胞的黏附和生长影响不同。目的:分析退火处理不同管径TiO2纳米管对成纤维细胞生物学行为的影响。方法:在5 V和20 V电压下分别采用阳极氧化方法在抛光纯钛表面制备TiO2纳米管,并行退火处理。将纯钛试样分为6组:抛光纯钛组、5 V纳米管组、20 V纳米管组、退火处理抛光纯钛组、退火处理5 V纳米管组、退火处理20V纳米管组,利用场发射扫描电镜观察试样表面形貌。各组试样表面接种成纤维细胞,采用胞核染色计数方法分析培养60,120 min时试样表面细胞的黏附数,利用扫描电镜观察培养1 d时的细胞形态,MTT法检测培养1,3,5 d后试样表面细胞的增殖情况,天狼星红苦味酸染色法分析培养3 d时细胞的胶原纤维分泌情况。结果与结论:①退火处理对钛表面TiO2纳米管形貌及管径无明显影响;②5,20 V纳米管组表面的细胞黏附数量少于抛光纯钛组;退火处理增加了抛光纯钛表面的成纤维细胞黏附数量,减少了5,20V纳米管表面的细胞黏附数量;③退火处理增强了抛光纯钛表面成纤维细胞的活性,降低了5,20 V纳米管表面的细胞活性;④5,20V纳米管组表面的细胞增殖活性低于抛光纯钛组;退火处理提高了抛光纯钛表面的细胞增殖活性,减少了5,20V纳米管表面的细胞增殖活性;⑤5,20V纳米管组表面细胞的胶原纤维分泌量高于抛光纯钛组;退火处理提高了抛光纯钛表面的细胞胶原分泌量,减少了5,20V纳米管表面的细胞胶原分泌量;⑥结果表明,TiO2纳米管不同程度地抑制成纤维细胞的黏附、伸展和增殖,退火处理可增强这种抑制作用;TiO2纳米管不同程度增强成纤维细胞的胶原分泌功能,退火处理抑制了这种增强效果。
BACKGROUND:Previous studies have shown that titanium nanotubes with different diameters affect the adhesion and growth of cells on the implant surface.OBJECTIVE:To investigate the effects of annealing treatment on the biological behaviors of fibroblasts on the surface of TiO2 nanotubes with different diameters.METHODS:TiO2 nanotubes were prepared by polishing pure titanium samples through anodizing at 5 V and 20 V respectively,and then annealed.The pure titanium samples were divided into six groups:P(polishing titanium),NT5(TiO2 nanotubes prepared at 5 V),NT20(TiO2 nanotubes prepared at 20 V),A-P(annealing treatment and polishing titanium),A-NT5(TiO2 nanotubes prepared at 5 V and annealed)and A-NT20(TiO2 nanotubes formed at 20 V and annealed).Field emission scanning electron microscopy was used to obsesrve the surface topography.Fibroblasts were inoculated on the surface of each group of samples.After 60 and 120 minutes of culture,the number of cells adhering to titanium sample surface was counted using cell nucleus staining method.On day 1 of culture,cell morphology was observed using scanning electron microscopy.On days 1,3 and 5 of culture,cell proliferation was detected by MTT method.On day 3 of culture,the secretion of collagen fiber was detected by picro-sirius red staining method.RESULTS AND CONCLUSION:(1)The annealing treatment had no significant effect on the morphology and diameter of the nanotubes.(2)The number of adherent cells in the NT5 and NT20 groups was significantly lower than that in the P group.The annealing treatment increased the number of fibroblasts on the surface of polished pure titanium and decreased the number of fibroblasts in the NT5 and NT20 groups.(3)Annealing enhanced the viability of fibroblasts on the surface of polished pure titanium and decreased the viability of cells on the surface of nanotubes prepared at 5 and 20 V.(4)Cell viability in the NT5 and NT20 groups was lower than that in the P group.Annealing treatment increased the viability of cells on the surface of polished
作者
李红彩
马壮
郭有玲
Li Hongcai;Ma Zhuang;Guo Youling(First Department of Stomatology,Zhuzhou Central Hospital,Zhuzhou 410002,Hunan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第22期3504-3509,共6页
Chinese Journal of Tissue Engineering Research
基金
湖南省卫生厅计划课题(c2013-051),项目负责人:马壮。
关键词
TiO2
纳米管
退火
成纤维细胞
细胞黏附
细胞增殖
细胞伸展
胶原分泌
TiO2
nanotubes
annealing treatment
fibroblasts
cell adhesion
cell proliferation
cell spreading
collagen secretion
