摘要
以7个灌木柳无性系为试验材料,采用水培法进行铅胁迫处理(Pb2+处理质量浓度分别为0,20,40和80 mg/L)后,分别测定并计算其生物量、耐性指数、毒性阈值、铅含量和转运系数,比较并分析无性系间对铅富集能力的差异。结果显示:各无性系之间及各处理之间,其生物量差异极显著;随着水培处理中铅质量浓度的升高,各无性系的耐性指数总体上表现为逐步降低的趋势,而无性系植株体内的铅含量总体上则表现为逐步升高的趋势;不同无性系铅富集能力差异明显,铅总量最高的无性系为P681[(7432.79±980.72)μg],最低的无性系为P646[(2990.64±335.59)μg];毒性阈值最高的无性系为P683(215.780 mg/L),最低的无性系为P646(22.911 mg/L)。认为无性系P61适合低质量浓度铅污染修复,无性系P63和P1024较适合高质量浓度铅污染修复,而无性系P646并不适合用来进行铅污染修复。
Pb tolerance and accumulation ability of 7 shrub willow clones was researched by hydroponic culture.Exposed in 0,20,40,80 mg/L Pb2+concentrations for 4 weeks,their biomass,tolerance indices(TI),critical toxicity thresholds(EC50),Pb content and translocation factor(TF)were determined.We found that in general,their tolerance and accumulation ability varied depending on Pb2+concentration.With the increase of Pb2+treatment concentration,their TI decreased but Pb content increased.There were significant differences in biomass among different clones and treatments.The highest total Pb content occurred in Clone P681[(7432.79+980.72)μg],and the lowest in Clone P646[(2990.64+335.59)μg].The highest EC50 was in Clone P683(215.780 mg/L)and the lowest was in Clone P646(22.911 mg/L).We concluded that Clone P61 was suitable for the bioremediation of low Pb2+concentration pollution while Clone P63 and P1024 more suitable for high Pb2+concentration pollution remediation,but Clone P646 was not suitable for bioremediation.
作者
黄瑞芳
王红玲
施士争
Huang Ruifang;Wang Hongling;Shi Shizheng(Jiangsu Academy of forestry,Nanjing 211153,China;The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China)
出处
《江苏林业科技》
2020年第1期11-16,22,共7页
Journal of Jiangsu Forestry Science & Technology
基金
江苏省农业科技自主创新资金项目“灌木柳树修复铅污染土壤的专用品种选育”[CX(19)3044]
国家科技支撑项目“黄淮海平原农区净水型农田林网防护系统构建技术研究与示范”(2015BAD07B0504)
中央财政林业科技推广项目“苏北杨树更新树种良种繁育及高效培育技术推广”(苏[2017]TG03号)
关键词
灌木柳树
无性系
铅
富集能力
重金属污染
生物修复
Shrub willow
Clone
Pb
Accumulation ability
Heavy metal pollution
Bioremediation
